Skip Navigation


JAC Advance Access originally published online on February 25, 2008
Journal of Antimicrobial Chemotherapy 2008 61(5):1003-1006; doi:10.1093/jac/dkn063
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
61/5/1003    most recent
dkn063v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Jiang, Y.
Right arrow Articles by Li, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jiang, Y.
Right arrow Articles by Li, L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Original research

Plasmid-mediated quinolone resistance determinants qnr and aac(6')-Ib-cr in extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae in China

Yan Jiang1,2, Zhihui Zhou1, Ying Qian1, Zeqing Wei1, Yunsong Yu1,*, Songnian Hu2,3 and Lanjuan Li1

1 State Key Laboratory for Diagnosis and Treatment of Infectious Disease, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China 2 James D. Watson Institute of Genome Sciences, Zhejiang University, Hangzhou, Zhejiang 310008, China 3 Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China

Received 22 October 2007; returned 20 November 2007; revised 25 January 2008; accepted 26 January 2008

* Corresponding author: Tel: +86-571-8723-6421; Fax: +86-571-8723-6423; E-mail: yvys119{at}163.com

Objectives: To characterize the prevalence of plasmid-mediated quinolone resistance determinants qnr and aac(6')-Ib-cr in extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae.

Methods: qnrA, qnrB, qnrS, aac(6')-Ib-cr and ESBL-encoding genes were detected by PCR. MICs of 10 antimicrobial agents were determined by Etest. PFGE was used to investigate the clonality of qnr- and aac(6')-Ib-cr-producing isolates. Conjugation and Southern hybridizations were used to confirm whether qnr, aac(6')-Ib-cr or ESBL-encoding genes were located on plasmids.

Results: Twenty-nine (8.0%) of 362 isolates were positive for qnr genes, and the qnrA-, qnrB- and qnrS-type genes were detected alone or in combination in 13 (3.6%), 8 (2.2%) and 9 (2.5%), respectively. Sixty-two (17.1%) isolates were positive for aac(6')-Ib, of which 36 (9.9% of all) had the -cr variant. Conjugation and Southern hybridization revealed that qnrA, aac(6')-Ib-cr and ESBL-encoding genes were always located on the same plasmids.

Conclusions: qnr and aac(6')-Ib-cr genes were detected in 8.0% and 9.9% of ESBL-producing E. coli and K. pneumoniae, respectively. The plasmids carrying the qnr gene could be transferred by conjugation together with ESBL-encoding genes and aac(6')-Ib-cr.

Keywords: conjugation , PFGE , Southern hybridization


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Clin. Microbiol. Rev.Home page
J. Strahilevitz, G. A. Jacoby, D. C. Hooper, and A. Robicsek
Plasmid-Mediated Quinolone Resistance: a Multifaceted Threat
Clin. Microbiol. Rev., October 1, 2009; 22(4): 664 - 689.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
M. Gunell, M. A. Webber, P. Kotilainen, A. J. Lilly, J. M. Caddick, J. Jalava, P. Huovinen, A. Siitonen, A. J. Hakanen, and L. J. V. Piddock
Mechanisms of Resistance in Nontyphoidal Salmonella enterica Strains Exhibiting a Nonclassical Quinolone Resistance Phenotype
Antimicrob. Agents Chemother., September 1, 2009; 53(9): 3832 - 3836.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
X.-Y. Pu, J.-C. Pan, H.-Q. Wang, W. Zhang, Z.-C. Huang, and Y.-M. Gu
Characterization of fluoroquinolone-resistant Shigella flexneri in Hangzhou area of China
J. Antimicrob. Chemother., May 1, 2009; 63(5): 917 - 920.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
M. Sjolund-Karlsson, J. P. Folster, G. Pecic, K. Joyce, F. Medalla, R. Rickert, and J. M. Whichard
Emergence of Plasmid-Mediated Quinolone Resistance among Non-Typhi Salmonella enterica Isolates from Humans in the United States
Antimicrob. Agents Chemother., May 1, 2009; 53(5): 2142 - 2144.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
G. A. Jacoby, N. Gacharna, T. A. Black, G. H. Miller, and D. C. Hooper
Temporal Appearance of Plasmid-Mediated Quinolone Resistance Genes
Antimicrob. Agents Chemother., April 1, 2009; 53(4): 1665 - 1666.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
J. Ma, Z. Zeng, Z. Chen, X. Xu, X. Wang, Y. Deng, D. Lu, L. Huang, Y. Zhang, J. Liu, et al.
High Prevalence of Plasmid-Mediated Quinolone Resistance Determinants qnr, aac(6')-Ib-cr, and qepA among Ceftiofur-Resistant Enterobacteriaceae Isolates from Companion and Food-Producing Animals
Antimicrob. Agents Chemother., February 1, 2009; 53(2): 519 - 524.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
H. B. Kim, C. H. Park, C. J. Kim, E.-C. Kim, G. A. Jacoby, and D. C. Hooper
Prevalence of Plasmid-Mediated Quinolone Resistance Determinants over a 9-Year Period
Antimicrob. Agents Chemother., February 1, 2009; 53(2): 639 - 645.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
P. Shen, Y. Jiang, Z. Zhou, J. Zhang, Y. Yu, and L. Li
Complete nucleotide sequence of pKP96, a 67 850 bp multiresistance plasmid encoding qnrA1, aac(6')-Ib-cr and blaCTX-M-24 from Klebsiella pneumoniae
J. Antimicrob. Chemother., December 1, 2008; 62(6): 1252 - 1256.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
G. Ll. Jones, R. E. Warren, S. J. Skidmore, V. A. Davies, T. Gibreel, and M. Upton
Prevalence and distribution of plasmid-mediated quinolone resistance genes in clinical isolates of Escherichia coli lacking extended-spectrum {beta}-lactamases
J. Antimicrob. Chemother., December 1, 2008; 62(6): 1245 - 1251.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.