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JAC Advance Access originally published online on December 21, 2007
Journal of Antimicrobial Chemotherapy 2008 61(2):453-455; doi:10.1093/jac/dkm500
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© The Author 2007. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Research letters

Identification of tet(S) gene area in tetracycline-resistant Streptococcus dysgalactiae subsp. equisimilis clinical isolates

Liang-Chun Liu1, Jui-Chang Tsai2,3, Po-Ren Hsueh4, Sung-Pin Tseng1, Wei-Chun Hung1, Hsiao-Jan Chen1 and Lee-Jene Teng1,4,*

1 Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, 1 Chang-Te Street, Taipei 100, Taiwan 2 Center for Optoelectronic Biomedicine, National Taiwan University College of Medicine, 1 Jen-Ai Road, Taipei 100, Taiwan 3 Division of Neurosurgery, Department of Surgery, National Taiwan University Hospital, Taipei 100, Taiwan 4 Department of Laboratory Medicine, National Taiwan University Hospital, Taipei 100, Taiwan

* Corresponding author. Tel: +886-2-23123456 ext. 6918; Fax: +886-2-23711574; E-mail: ljteng@ntu.edu.tw

Keywords: IS1216 , truncated tnpA , chromosome location

The first 10% of the full text of this article appears below.

Sir,

While screening the association of IS1216 with antibiotic resistance genes using primers IS1216F(55)1 and IS1216_upR (5'-CATCGTCATTCCTCCTGCTATC-3'), we found a 4.5 kb product from a tetracycline-resistant Streptococcus dysgalactiae subsp. equisimilis clinical isolate, NTUH-3089. DNA sequencing revealed the presence of the tet(S) gene flanked by IS1216 and other open reading frames (ORFs). Since the tet(S) gene has not been previously reported in this species, we further analysed the presence and the genetic environment of tet(S) in clinical isolates of S. dysgalactiae subsp. equisimilis.

First, we screened the presence of tet(S) by PCR from 188 . . . [Full Text of this Article]


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