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JAC Advance Access originally published online on June 26, 2007
Journal of Antimicrobial Chemotherapy 2007 60(3):702-703; doi:10.1093/jac/dkm239
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© The Author 2007. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Correspondence

Learning from mistakes: Taq polymerase contaminated with ß-lactamase sequences results in false emergence of Streptococcus pneumoniae containing TEM

Raffaella Koncan1, Aránzazu Valverde2, María-Isabel Morosini2, María García-Castillo2, Rafael Cantón2, Giuseppe Cornaglia1, Fernando Baquero2 and Rosa del Campo2,*

1 Dipartimento di Patologia, Sezione di Microbiologia, Università degli Studi di Verona, Strada Le Grazie 8, 37134 Verona, Italy 2 Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Ctra Colmenar Km 9.1, Madrid 28034, Spain

* Corresponding author. Tel: +34-91-3368542; Fax: +34-91-3368809; E-mail: rosacampo@yahoo.com

Keywords: S. pneumoniae , contamination

The first 10% of the full text of this article appears below.

Sir,

In 2004, an Asiatic group published an article entitled ‘Study on the molecular epidemiology of ß-lactamase TEM gene in isolated Streptococcus pneumoniae’.1 This article can be found in the NCBI web site with the PMID number 15769331. The original article was written in Chinese, but in the published abstract, the authors reflected that 91.3% of their isolates had positive PCR amplifications for TEM. After sequencing these amplicons, the authors . . . [Full Text of this Article]

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