JAC Advance Access originally published online on April 25, 2007
Journal of Antimicrobial Chemotherapy 2007 60(1):20-41; doi:10.1093/jac/dkm110
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© The Author 2007. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
BSAC standardized disc susceptibility testing method (version 6)
Department of Microbiology, City Hospital NHS Trust, Birmingham B18 7QH, UK
* Corresponding author. Tel: +44-121-507-5693; Fax: +44-121-507-5521; E-mail: jenny.andrews@swbh.nhs.uk
Received 7 March 2007; returned 12 March 2007; revised 14 March 2007; accepted 16 March 2007
Keywords: breakpoints , disc testing , MICs
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Preface |
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Since the Journal of Antimicrobial Chemotherapy Supplement containing the British Society for Antimicrobial Chemotherapy (BSAC) standardized disc susceptibility testing method was published in 2001, there have been various changes to the recommendations and these have been posted on the BSAC website (http://www.bsac.org.uk). One major organizational change has been the harmonization of MIC breakpoints in Europe.
In 2002, the BSAC agreed to participate with several other European national susceptibility testing committees, namely CA-SFM (Comité de l'Antibiogramme de la Société Française de Microbiologie, France), the CRG (Commissie Richtlijnen Gevoeligheidsbepalingen, The Netherlands), DIN (Deutsches Institut für Normung, Germany), NWGA (Norwegian Working Group on Antimicrobials, Norway) and the SRGA (Swedish Reference Group of Antibiotics, Sweden), in a project to harmonize antimicrobial breakpoints, including previously established values that varied among countries. This work is being undertaken by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) with the support and collaboration
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Introduction |
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1. Preparation of plates |
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2. Selection of control organisms |
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3. Preparation of inoculum |
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3.1 Comparison with 0.5 McFarland standard
3.1.1 Preparation of the McFarland standard. 3.1.2 Inoculum preparation by the growth method (for non-fastidious organisms, e.g. Enterobacteriaceae, Pseudomonas spp. and staphylococci). 3.1.3 Inoculum preparation by the direct colony suspension method (the method of choice for fastidious organisms, i.e. Haemophilus spp., Neisseria gonorrhoeae, Neisseria meningitidis, Moraxella catarrhalis, Streptococcus pneumoniae, 
- and ß-haemolytic streptococci, Clostridium perfringens, Bacteroides fragilis, Bacteroides thetaiotaomicron, Campylobacter spp., Pasteurella multocida and Coryneform organisms). 3.1.4 Adjustment of the organism suspension to the density of the 0.5 McFarland standard. 3.2 Dilution of suspension adjusted to the turbidity of a 0.5 McFarland standard
3.3 Photometric standardization of turbidity of suspensions
3.4 Direct susceptibility testing
3.4.1 Direct susceptibility testing of urines. 3.4.2.1 Direct susceptibility testing of positive blood cultures
3.4.2.2 Gram-negative bacilli
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4. Inoculation of agar plates |
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4.2 Use of rotary platers for susceptibility testing
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5. Antimicrobial discs |
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5.2 Storage and handling of discs
5.3 Application of discs
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6. Incubation |
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6.2 Conditions of incubation
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7. Measuring zones and interpretation |
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7.1 Acceptable inoculum density
7.2 Measuring zones
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Transparency declarations |
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Appendix 1 |
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Testing antimicrobial susceptibility to co-trimoxazole
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Appendix 2 |
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Efficacy of cefaclor in the treatment of respiratory infections caused by H. influenzae
Cefaclor pharmacokinetics. Cefaclor potency against H. influenzae. Pharmacodynamics. Conclusion.
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Appendix 3 |
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1. Susceptibility testing of Helicobacter pylori. 2. Susceptibility testing of Brucella species. 3. Susceptibility testing of Legionella species.
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