Skip Navigation


JAC Advance Access originally published online on March 6, 2007
Journal of Antimicrobial Chemotherapy 2007 59(4):739-741; doi:10.1093/jac/dkm020
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
59/4/739    most recent
dkm020v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Tsakris, A.
Right arrow Articles by Pournaras, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tsakris, A.
Right arrow Articles by Pournaras, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Characterization of In3Mor, a new integron carrying VIM-1 metallo-ß-lactamase and sat1 gene, from Morganella morganii

Athanassios Tsakris1,*, Alexandros Ikonomidis2, Nicholas Spanakis1, Aggeliki Poulou3 and Spyros Pournaras2

1 Department of Microbiology, Medical School, University of Athens, Athens, Greece 2 Department of Microbiology, Medical School, University of Thessaly, Larissa, Greece 3 Department of Microbiology, General Hospital of Serres, Serres, Greece

Received 19 November 2006; returned 20 December 2006; revised 27 December 2006; accepted 11 January 2007

* Corresponding author. Tel: +30-210-746-2010; Fax: +30-210-746-2249; E-mail: atsakris{at}med.uoa.gr

Objectives: A carbapenem-resistant Morganella morganii clinical isolate that was phenotypically metallo-ß-lactamase (MBL)-positive was recovered from a Greek patient. The aim of the study was to analyse the structure of the integron containing the MBL gene.

Methods: MICs were determined by the broth microdilution method. PCR assays and nucleotide sequencing were used for identification of bla gene types and mapping of the integron carrying the MBL gene. The location of the MBL allele was investigated by mating experiments and plasmid analysis as well as by Southern blotting of the plasmid extract and gene-specific hybridization with a blaVIM-1 probe.

Results: The strain contained In3Mor, a novel class 1 integron carrying a carbapenemase gene (blaVIM-1) associated with a trimethoprim (dfrA1), a streptothricin (sat1) and two aminoglycoside resistance genes (aacA7 and aadA1). Conjugation experiments failed to detect a transferable MBL determinant and plasmid DNA was not visualized. The chromosomal location of blaVIM-1 was confirmed after hybridization of the chromosomal band with the blaVIM-1 probe.

Conclusions: Production of a VIM-type MBL in a M. morganii clinical isolate is documented in this study for the first time. Also, the dfrA1-sat1-aadA1 array which is typically described in the variable region of class 2 integrons consistent with that on Tn7 transposons, is originally detected herein in a class 1 integron.

Keywords: M. morganii , resistance , carbapenems , MBLs


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
C. Juan, A. Beceiro, O. Gutierrez, S. Alberti, M. Garau, J. L. Perez, G. Bou, and A. Oliver
Characterization of the New Metallo-{beta}-Lactamase VIM-13 and Its Integron-Borne Gene from a Pseudomonas aeruginosa Clinical Isolate in Spain
Antimicrob. Agents Chemother., October 1, 2008; 52(10): 3589 - 3596.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.