The tetracycline resistance determinant Tet 39 and the sulphonamide resistance gene sulII are common among resistant Acinetobacter spp. isolated from integrated fish farms in Thailand

doi:10.1093/jac/dkl419

JAC Advance Access originally published online on November 9, 2006
Journal of Antimicrobial Chemotherapy 2007 59(1):23-27; doi:10.1093/jac/dkl419

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© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

The tetracycline resistance determinant Tet 39 and the sulphonamide resistance gene sulII are common among resistant Acinetobacter spp. isolated from integrated fish farms in Thailand

Yvonne Agersø¹^,* and Andreas Petersen²

¹ Danish Institute for Food and Veterinary Research Bülowsvej 27, DK-1790 Copenhagen V, Denmark ² Department of Veterinary Pathobiology, The Royal Veterinary and Agricultural University Stigbøjlen 4, DK-1870 Frederiksberg C, Denmark

Received 29 May 2006; returned 19 July 2006; revised 16 September 2006; accepted 18 September 2006

^*Corresponding author. Tel: +45-72-34-6273; Fax: +45-72-34-6001; E-mail: ya{at}dfvf.dk

Objectives: To determine the genetic basis for tetracyclineand sulphonamide resistance and the prevalence of class I andII integrons in oxytetracycline-resistant Acinetobacter spp.from integrated fish farms in Thailand.

Methods: A total of 222 isolates were screened for tetracyclineresistance genes [tet(A), tet(B), tet(H), tet(M) and tet(39)]and class II integrons by PCR. One hundred and thirty-four ofthese isolates were also sulphonamide resistant and these isolateswere screened for sulphonamide resistance genes (sulII and sulIII)as well as class I integrons. Plasmid extraction and Southernblots with sulII and tet(39) probes were performed on selectedisolates.

Results: The recently identified tetracycline resistance genetet(39) was demonstrated in 75% (166/222) of oxytetracycline-resistantAcinetobacter spp. from integrated fish farms in Thailand. Isolatesthat were also sulfamethoxazole-resistant contained sulII (96%;129/134) and/or sulI (14%; 19/134) (as part of class I integrons).sulII and tet(39) were located on plasmids differing in sizein the isolates tested.

Conclusions: The study shows tet(39) and sulII to be commonresistance genes among clonally distinct Acinetobacter spp.from integrated fish farms and these bacteria may constitutereservoirs of resistance genes that may increase owing to aselective pressure caused by the use of antimicrobials in theoverlaying animal production.

Keywords: aquatic environments , integrons , tet(39) , tetA(39)

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