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JAC Advance Access originally published online on October 23, 2006
Journal of Antimicrobial Chemotherapy 2006 58(6):1254-1256; doi:10.1093/jac/dkl418
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© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Fusidic acid resistance, mediated by fusB, in bovine coagulase-negative staphylococci

Siamak P. Yazdankhah1,*, Anette W. Åsli1, Henning Sørum1, Hanne Oppegaard2 and Marianne Sunde3

1 Department of Food Safety and Infection Biology, The Norwegian School of Veterinary Science Oslo, Norway 2 TINE Norwegian Dairies BA, R&D Centre Oslo, Norway 3 Section of Bacteriology, National Veterinary Institute Oslo, Norway

Received 22 June 2006; returned 25 July 2006; revised 18 September 2006; accepted 18 September 2006

*Correspondence address. Norwegian Scientific Committee for Food Safety, Norwegian Institute of Public Health, PO Box 4404 Nydalen, N-0403 Oslo, Norway. Tel: +47-21-62-28-09; Fax +47-21-62-28-01; E-mail: siamak.yazdankhah{at}fhi.no

Objectives: The aim of this study was to determine the occurrence of fusidic acid resistance, mediated by the fusB gene, among coagulase-negative staphylococci (CoNS) isolated from bovine mastitis.

Methods: A total of 113 CoNS isolates were screened for susceptibility to fusidic acid by using a disc diffusion method. The fusB gene was detected by using PCR and subsequent DNA sequencing. The localization of fusB was determined by hybridization.

Results: The fusB gene was detected in 3 of 11 fusidic acid-resistant bovine CoNS isolates. The organization of the fusB downstream region on a 40 kb plasmid in a Staphylococcus haemolyticus isolate (288/96) was highly similar to the previously reported organization of fusB on plasmid pUB101 from a Staphylococcus aureus isolate of human origin. The fusB gene was chromosomally located in the remaining two isolates.

Conclusions: Fusidic acid resistance mediated by fusB is not the dominant resistance mechanism in fusidic acid-resistant CoNS studied in this work. The similarity between the organization of the fusB downstream region in S. haemolyticus (isolate 288/96) and on plasmid pUB101 from an S. aureus isolate of human origin indicates a common ancestral origin of these genes.

Keywords: bovine mastitis , plasmid , S. haemolyticus


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