JAC Advance Access originally published online on July 1, 2006
Journal of Antimicrobial Chemotherapy 2006 58(3):530-536; doi:10.1093/jac/dkl266
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Characterization of acquired ß-lactamases and their genetic support in multidrug-resistant Pseudomonas aeruginosa isolates in Taiwan: the prevalence of unusual integrons
1 Departments of Pathology, College of Medicine, National Cheng Kung University No. 1 University Road, Tainan 70101, Taiwan 2 Internal Medicine, College of Medicine, National Cheng Kung University No. 1 University Road, Tainan 70101, Taiwan 3 Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University No. 1 University Road, Tainan 70101, Taiwan 4 Department of Laboratory Medicine, National Taiwan University Hospital No.7 Chung San South Road, Taipei 10002, Taiwan 5 Departments of Pathology, Tri-service General Hospital Sec. 2, 325 Chenggong Road, Taipei 11490, Taiwan 6 Internal Medicine, Tri-service General Hospital Sec. 2, 325 Chenggong Road, Taipei 11490, Taiwan
Received 10 March 2006; returned 2 May 2006; revised 19 May 2006; accepted 1 June 2006
*Corresponding author. Tel: +886-6-2353535 ext. 5605; Fax: +886-6-2363956; E-mail: jjwu{at}mail.ncku.edu.tw
Objectives: The present study was conducted to investigate acquired ß-lactamases and their genetic support in 26 Pseudomonas aeruginosa isolates that were resistant to nearly all antipseudomonal drugs from six medical centres in Taiwan.
Methods: Acquired ß-lactamases and their genetic support were determined by PCR-based strategies.
Results: Four and 16 of the 26 isolates were found to produce VIM-2 and VIM-3 metallo-ß-lactamases (MBLs), respectively, and 1, 1 and 2 isolates produced OXA-17, OXA-10 and PSE-1, respectively. These bla genes are all in class 1 integrons that are probably chromosomally located. The blaVIM-3-containing integron, with a deletion between int1 and the blaVIM-3 structural gene, has six gene cassettes, blaVIM-3, a probable fosfomycin resistance determinant, aacA4, aacA4, aadB and aacA4. The blaVIM-2-containing integron, without detectable 5'-conserved segment, contains four genes cassettes (aacA7-blaVIM-2-dhfr-aacA5) and is ended by tniC. The blaOXA-10-containing integron includes a catB3 cassette and a fused gene cassette, which is made up of blaOXA-17 and a novel streptomycin–spectinomycin gene, designated aadA15. The blaOXA-17-containing integron has three gene cassettes (aacA4-catB2-blaOXA-17) but the 59-base element of the blaOXA-17 cassette is interrupted by a putative transposase gene. The blaPSE-1-containing integron has three gene cassettes, aacA4, an aadA3-related gene designated aadA3b and blaPSE-1. PFGE revealed genetic diversity among the multidrug-resistant isolates from different hospitals.
Conclusions: This study demonstrated the high prevalence of VIM-type MBLs and the presence of unusual bla-encoding integrons in multidrug-resistant P. aeruginosa isolates in Taiwan. The spread of blaVIM-2-related genes by horizontal transfer might have occurred.
Keywords: carbapenemases , penicillinases , gene cassettes , fosfomycin
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