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JAC Advance Access originally published online on June 2, 2006
Journal of Antimicrobial Chemotherapy 2006 58(2):432-433; doi:10.1093/jac/dkl240
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© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Molecular characterization of an Escherichia coli clinical isolate that produces both metallo-ß-lactamase VIM-2 and extended-spectrum ß-lactamase GES-7: identification of the In8 integron carrying the blaVIM-2 gene

Irene Galani, Maria Souli, Evangelos Koratzanis, Zoi Chryssouli and Helen Giamarellou*

4th Department of Internal Medicine, Molecular Biology Section, University General Hospital ‘ATTIKON’ Rimini 1, 124 62 Chaidari, Greece

Received 8 February 2006; returned 7 April 2006; revised 15 May 2006; accepted 15 May 2006

*Corresponding author. Tel: +30-2105326426; Fax: +30-2105326446; E-mail: hgiama{at}ath.forthnet.gr

Objectives: We have previously reported the isolation of a VIM-2-producing Escherichia coli clinical isolate and the coexistence of blaVIM-2 and blaGES-7 in the same isolate. The aim of this study was to elucidate the genetic environment of these genes.

Methods: PCR and sequence analysis were used to identify and analyse the blaVIM-containing integron. The location of the blaVIM-2 and blaGES-7 genes was identified by hybridization experiments on I-CeuI-digested genomic DNA after PFGE.

Results: Only the blaVIM-2 gene has been found on a class I integron, designated In8 (1474 bp). Hybridization with the blaVIM-2, the blaGES-7 and the 16S–23S rRNA probes confirmed the chromosomal location of both genes. The blaVIM-2 probe hybridized with a 710 kb fragment whereas the blaGES-7 probe hybridized with a 144 kb fragment of genomic DNA of the E. coli isolate.

Conclusions: This report provides evidence for the chromosomal location of both blaVIM-2 and blaGES-7 genes carried by an E. coli clinical isolate. In8, containing blaVIM-2, presents an emerging threat of carbapenem resistance among E. coli isolates in Greece.

Keywords: E. coli , blaVIM-2 , blaGES-7


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