JAC Advance Access originally published online on September 2, 2005
Journal of Antimicrobial Chemotherapy 2005 56(4):698-702; doi:10.1093/jac/dki324
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Dissemination of SHV-12 and CTX-M-type extended-spectrum ß-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae and emergence of GES-3 in Korea
1 Department of Laboratory Medicine, Keimyung University College of Medicine, Daegu; 2 Seoul National University College of Medicine, Seoul; 3 Pochon CHA University College of Medicine, Sungnam; 4 The Catholic University of Korea College of Medicine, Seoul; 5 Department of Laboratory Medicine and 6 Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul; and 7 Department of Laboratory Medicine, Kosin University College of Medicine, Busan, Korea
Received 26 April 2005; returned 5 June 2005; revised 15 August 2005; accepted 15 August 2005
* Correspondence address. Department of Laboratory Medicine, Kosin University College of Medicine, 34 Amnam Dong, Suh Gu, Busan, Korea, 602–703. Tel: +82-51-990-6373; Fax: +82-51-990-3034; E-mail: kscpjsh{at}ns.kosinmed.or.kr
Objectives: To assess the prevalence and genotypes of Ambler class A extended-spectrum ß-lactamases (ESBLs) in Korea.
Methods: Clinical isolates of Escherichia coli and Klebsiella pneumoniae collected from 12 Korean hospitals during February–July 2003 were evaluated. Antimicrobial susceptibilities were determined by disc diffusion and agar dilution methods, and the putative ESBL-producing strains were tested by the double-disc synergy method. Detection of genes encoding class A ß-lactamases was performed by PCR amplification, and the PCR products were subjected to direct sequencing.
Results: The double-disc synergy test showed positive results in 9.3% (23/246) of E. coli and 23.0% (55/239) of K. pneumoniae isolates. The most prevalent types of Ambler class A ESBLs in E. coli isolates were CTX-M-15 (n = 4) and CTX-M-3 (n = 3), and those in K. pneumoniae isolates were SHV-12 (n = 30) and CTX-M-3 (n = 13). Two isolates produced both SHV-12 and GES-3, simultaneously.
Conclusions: CTX-M-type and/or SHV-12 ESBL-producing E. coli and K. pneumoniae isolates are spreading, and a GES-type ESBL has emerged in Korea.
Keywords: ESBLs , resistance , prevalence
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