JAC Advance Access originally published online on June 7, 2005
Journal of Antimicrobial Chemotherapy 2005 56(1):228-231; doi:10.1093/jac/dki193
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The antibacterial activity of honey against coagulase-negative staphylococci
1 Honey Research Unit, Department of Biological Sciences University of Waikato, Private Bag 3105, Hamilton, New Zealand; 2 Centre for Biomedical Sciences, School of Applied Sciences, University of Wales Institute, Cardiff, UK
Received 22 February 2005; returned 31 March 2005; revised 12 May 2005; accepted 15 May 2005
* Corresponding author. Tel: +64-7-8384325; Fax: +64-7-8384324; E-mail: pmolan{at}waikato.ac.nz
Objectives: Development of antibiotic-resistant strains of coagulase-negative staphylococci has complicated the management of infections associated with the use of invasive medical devices, and innovative treatment and prophylactic options are needed. Honey is increasingly being used to treat infected wounds, but little is known about its effectiveness against coagulase-negative staphylococci. The aim of this study was to determine the minimum active dilution of two standardized, representative honeys for 18 clinical isolates of coagulase-negative staphylococci.
Methods: An agar incorporation technique was used to determine the minimum active dilution, with dilution steps of 1% (v/v) honey [or steps of 5% (v/v) of a sugar syrup matching the osmotic effect of honey]. The plates were inoculated with 10 µL spots of cultures of the isolates.
Results: The honeys were inhibitory at dilutions down to 3.6 ± 0.7% (v/v) for the pasture honey, 3.4 ± 0.5% (v/v) for the manuka honey and 29.9 ± 1.9% (v/v) for the sugar syrup.
Conclusions: Typical honeys are about eight times more potent against coagulase-negative staphylococci than if bacterial inhibition were due to their osmolarity alone. Therefore, honey applied to skin at the insertion points of medical devices may have a role in the treatment or prevention of infections by coagulase-negative staphylococci.
Keywords: invasive medical devices , antibiotic resistance , minimum active dilution , manuka honey , device-related infections
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