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JAC Advance Access originally published online on May 4, 2005
Journal of Antimicrobial Chemotherapy 2005 55(6):832-839; doi:10.1093/jac/dki118
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© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions{at}oupjournals.org

Genetic analysis of pbp2x in clinical Streptococcus pneumoniae isolates in Quebec, Canada

Dominic Granger1,2, Geneviève Boily-Larouche1, Pierre Turgeon2,3, Karl Weiss2,4 and Michel Roger1,2,*

1 Laboratoire d'immunogénétique, Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CHUM), Montréal, Canada; 2 Département de microbiologie-immunologie, Hôpital Notre-Dame du CHUM, 1560 Sherbrooke Est, Montréal, Québec, Canada H2L 4M1; 3 Département de microbiologie, Hôpital Saint-Luc du CHUM, Montréal, Canada; 4 Département de microbiologie, Hôpital Maisonneuve-Rosemont, Montréal, Canada

* Corresponding author. Tel: +1-514-890-8000, ext. 25802; Fax: +1-514-412-7512; Email: michel.roger{at}ssss.gouv.qc.ca

Objectives: To investigate the nature of the amino acid motifs found in penicillin-binding protein (PBP) 2x of penicillin-resistant Streptococcus pneumoniae isolates across the province of Quebec (Canada), and to obtain preliminary information regarding the prevalence of these alterations.

Methods: The pbp2x genomic region encompassing codons 178–703, which includes the entire region of the transpeptidase domain, was sequenced and compared for 52 clinical isolates comprising 20 penicillin-susceptible (PSSP), 20 penicillin-intermediate (PISP) and 12 penicillin-resistant (PRSP) pneumococci.

Results: The degree of diversity within PBP2x correlated with increased resistance to ß-lactam antibiotics. There were an average of 5.0 ± 1.8 mutations in PSSP, 37.9 ± 4.4 in PISP, and 63.0 ± 2.0 in PRSP isolates when compared with the control penicillin-susceptible strain R6. At least six distinct amino acid profiles were identified among PISP strains isolated in Quebec. In contrast, all PRSP isolates shared a similar pattern of altered amino acids compared with the sequence from susceptible strains.

Conclusions: These data will be useful in future studies to monitor the genetic changes associated with the emergence and spread of ß-lactam resistance in Quebec.

Keywords: penicillin-binding proteins , ß-lactams , pneumococci , serotype , penicillin resistance , cefotaxime resistance


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