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JAC Advance Access originally published online on March 2, 2005
Journal of Antimicrobial Chemotherapy 2005 55(4):461-465; doi:10.1093/jac/dkh556
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© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions{at}oupjournals.org

Description of In116, the first blaCTX-M-2-containing complex class 1 integron found in Morganella morganii isolates from Buenos Aires, Argentina

Pablo Power1, Moreno Galleni2, José Di Conza1, Juan A. Ayala3 and Gabriel Gutkind1,*

1 Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 (1113), Buenos Aires, Argentina; 2 Centre d'Ingéniérie des Protéines, Université de Liège, Institut de Chimie, B6 (B-4000), Sart Tilman, Belgium; 3 Centro de Biología Molecular ‘Severo Ochoa’, CSIC-UAM, Campus de Cantoblanco (28049), Madrid, Spain

* Corresponding author. Email: ggutkind{at}ffyb.uba.ar

Objectives: We analysed the architecture and probable origin of a class 1 integron from cefotaxime-resistant Morganella morganii isolates.

Methods: bla genes and class 1 integron elements were detected by PCR and DNA–DNA hybridization in a M. morganii strain isolated in 1996. PCR-mapping and sequencing of different fragments were carried out to determine the integron's architecture.

Results and conclusions: A class 1 integron (In116), strongly related to the In6/In7 family, was detected in a plasmid from an oxyimino-cephalosporin-resistant M. morganii strain, producing CTX-M-2 ß-lactamase. The variable region of In116 contains aacA4, blaOXA-2 and orfD cassettes. Downstream of the 3'-conserved-segment (3'-CS), an orf513-containing common region is followed by blaCTX-M-2 and flanking regions, having 96–99% nucleotide identity with Kluyvera ascorbata's kluA-1 and neighbouring sequences. Some of the evidence supporting the incorporation of foreign DNA is as follows: a partial deletion in a second 3'-CS (3'-CS2), and the absence of 59-base element or IS-like structures upstream of blaCTX-M-2.

Keywords: Kluyvera ascorbata , In6/In7 , orf513 , aacA4 , blaOXA-2


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