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JAC Advance Access originally published online on January 13, 2005
Journal of Antimicrobial Chemotherapy 2005 55(2):272-274; doi:10.1093/jac/dkh545
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JAC vol.55 no.2 © The British Society for Antimicrobial Chemotherapy 2005; all rights reserved

In vitro activity and killing effect of temporin A on nosocomial isolates of Enterococcus faecalis and interactions with clinically used antibiotics

Andrea Giacometti1, Oscar Cirioni1, Wojciech Kamysz2, Giuseppina D'Amato1, Carmela Silvestri1, Maria Simona Del Prete1, Alberto Licci1, Jerzy Lukasiak2 and Giorgio Scalise1

1 Institute of Infectious Diseases and Public Health, Università Politecnica delle Marche, Ancona, Italy; 2 Faculty of Pharmacy, Medical University of Gdansk, Gdansk, Poland

* Corresponding author. Tel: +39-071-5963715; Fax: +39-071-5963468; Email: anconacmi{at}interfree.it

Objective: To study the in vitro activity of temporin A, a basic, highly hydrophobic, antimicrobial peptide amide derived from the skin of the European red frog Rana temporaria, alone and in combination with co-amoxiclav, imipenem, ciprofloxacin, linezolid and vancomycin, against 42 nosocomial isolates of Enterococcus faecalis. Fourteen of these were resistant to vancomycin.

Methods: Antimicrobial activity of temporin A was measured by MIC, MBC and time–kill studies and by the chequerboard titration method.

Results: All isolates were inhibited at concentrations of 1 to 16 mg/L. Combination studies carried out with E. faecalis ATCC 29212 and ATCC 51299 demonstrated synergy only when the peptide was combined with co-amoxiclav and imipenem.

Conclusions: Our findings show that temporin A is active against E. faecalis and that its activity could be enhanced when it is combined with other antimicrobial agents.

Keywords: antimicrobial peptides , Gram-positive infections , susceptibility , antibiotic combinations , enterococci


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