Skip Navigation


JAC Advance Access originally published online on October 14, 2004
Journal of Antimicrobial Chemotherapy 2004 54(6):1007-1012; doi:10.1093/jac/dkh455
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
54/6/1007    most recent
dkh455v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (23)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Yan, J.-J.
Right arrow Articles by Li, J.-D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yan, J.-J.
Right arrow Articles by Li, J.-D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

JAC vol.54 no.6 © The British Society for Antimicrobial Chemotherapy 2004; all rights reserved

Plasmid-mediated 16S rRNA methylases conferring high-level aminoglycoside resistance in Escherichia coli and Klebsiella pneumoniae isolates from two Taiwanese hospitals

Jing-Jou Yan1,2,*, Jiunn-Jong Wu3, Wen-Chien Ko4, Shu-Huei Tsai2, Chin-Luan Chuang2, Hsiu-Mei Wu3, Ying-Jiun Lu1 and Jau-Dai Li5

1Department of Pathology, National Cheng Kung University Hospital, No. 138, Sheng-Li Rd, Tainan 70428; Departments of 2 Pathology, 3 Medical Technology and 4 Internal Medicine, College of Medicine, National Cheng Kung University, No. 1, University Rd, Tainan 70101; 5 Department of Laboratory Medicine, Tainan Municipal Hospital, No. 670, Chongde Rd, Tainan City 70173, Taiwan

* Corresponding author. Tel: +886-6-235-3535; Fax: +886-6-276-6195; Email: jingjou{at}mail.ncku.edu.tw

Objectives: This study was conducted to investigate the occurrence of 16S rRNA methylases that confer high-level aminoglycoside resistance in Klebsiella pneumoniae and Escherichia coli isolates from two Taiwanese hospitals and the characteristics of these isolates.

Methods: A total of 1624 K. pneumoniae and 2559 E. coli isolates consecutively collected over an 18 month period from a university hospital and seven E. coli and eight K. pneumoniae isolates that were resistant to amikacin from a district hospital were analysed. Two 16S rRNA methylase genes, armA and rmtB, were detected by PCR-based assays. ß-Lactamase characteristics were determined by phenotypic and genotypic methods.

Results: Overall, 28 armA-positive and seven rmtB-positive isolates were identified, and extended-spectrum ß-lactamases (ESBLs) were detected in 33 (94.3%) isolates. The prevalence rates of armA and rmtB at the university hospital were 0.9% (n=15) and 0.3% (n=5) in K. pneumoniae and 0.4% (n=10) and 0.04% (n=1) in E. coli. CTX-M-3, CTX-M-14, SHV-5-like ESBLs, and CMY-2 were detected alone or in combination in 21, 6, 11, and 2, respectively, of the 28 armA-positive isolates. CTX-M-14 was detected in six of the seven rmtB-positive isolates. Fingerprinting of conjugative plasmids revealed the dissemination of closely related plasmids containing both armA and blaCTX-M-3. PFGE suggests that armA and rmtB spread by both horizontal transfer and clonal spread.

Conclusions: This is the first report of the emergence of 16S rRNA methylases in Enterobacteriaceae in Taiwan. The spread of the multidrug-resistant isolates producing both ESBLs and 16S rRNA methylases may become a clinical problem.

Keywords: resistance genes , multidrug resistance , armA , rmtB , extended-spectrum ß-lactamases


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
L. Ma, C.-J. Lin, J.-H. Chen, C.-P. Fung, F.-Y. Chang, Y.-K. Lai, J.-C. Lin, L. K. Siu, and and the Taiwan Surveillance of Antimicrobial Resis
Widespread Dissemination of Aminoglycoside Resistance Genes armA and rmtB in Klebsiella pneumoniae Isolates in Taiwan Producing CTX-M-Type Extended-Spectrum {beta}-Lactamases
Antimicrob. Agents Chemother., January 1, 2009; 53(1): 104 - 111.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
Q. Wu, Y. Zhang, L. Han, J. Sun, and Y. Ni
Plasmid-Mediated 16S rRNA Methylases in Aminoglycoside-Resistant Enterobacteriaceae Isolates in Shanghai, China
Antimicrob. Agents Chemother., January 1, 2009; 53(1): 271 - 272.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
M. D. Tamang, S. Y. Seol, J.-Y. Oh, H. Y. Kang, J. C. Lee, Y. C. Lee, D. T. Cho, and J. Kim
Plasmid-Mediated Quinolone Resistance Determinants qnrA, qnrB, and qnrS among Clinical Isolates of Enterobacteriaceae in a Korean Hospital
Antimicrob. Agents Chemother., November 1, 2008; 52(11): 4159 - 4162.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
Y. Zhang, H. Zhou, X.-q. Shen, P. Shen, Y.-s. Yu, and L.-j. Li
Plasmid-borne armA methylase gene, together with blaCTX-M-15 and blaTEM-1, in a Klebsiella oxytoca isolate from China
J. Med. Microbiol., October 1, 2008; 57(10): 1273 - 1276.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
Y. Doi, J. M. Adams-Haduch, and D. L. Paterson
Escherichia coli Isolate Coproducing 16S rRNA Methylase and CTX-M-Type Extended-Spectrum {beta}-Lactamase Isolated from an Outpatient in the United States
Antimicrob. Agents Chemother., March 1, 2008; 52(3): 1204 - 1205.
[Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
Z. Zong, S. R. Partridge, and J. R. Iredell
RmtC 16S rRNA Methyltransferase in Australia
Antimicrob. Agents Chemother., February 1, 2008; 52(2): 794 - 795.
[Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
H. Y. Kang, K. Y. Kim, J. Kim, J. C. Lee, Y. C. Lee, D. T. Cho, and S. Y. Seol
Distribution of Conjugative-Plasmid-Mediated 16S rRNA Methylase Genes among Amikacin-Resistant Enterobacteriaceae Isolates Collected in 1995 to 1998 and 2001 to 2006 at a University Hospital in South Korea and Identification of Conjugative Plasmids Mediating Dissemination of 16S rRNA Methylase
J. Clin. Microbiol., February 1, 2008; 46(2): 700 - 706.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
J.-i. Wachino, K. Shibayama, H. Kurokawa, K. Kimura, K. Yamane, S. Suzuki, N. Shibata, Y. Ike, and Y. Arakawa
Novel Plasmid-Mediated 16S rRNA m1A1408 Methyltransferase, NpmA, Found in a Clinically Isolated Escherichia coli Strain Resistant to Structurally Diverse Aminoglycosides
Antimicrob. Agents Chemother., December 1, 2007; 51(12): 4401 - 4409.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
B. Perichon, P. Courvalin, and M. Galimand
Transferable Resistance to Aminoglycosides by Methylation of G1405 in 16S rRNA and to Hydrophilic Fluoroquinolones by QepA-Mediated Efflux in Escherichia coli
Antimicrob. Agents Chemother., July 1, 2007; 51(7): 2464 - 2469.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
Y. Doi, D. de Oliveira Garcia, J. Adams, and D. L. Paterson
Coproduction of Novel 16S rRNA Methylase RmtD and Metallo-{beta}-Lactamase SPM-1 in a Panresistant Pseudomonas aeruginosa Isolate from Brazil
Antimicrob. Agents Chemother., March 1, 2007; 51(3): 852 - 856.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
P. Bogaerts, M. Galimand, C. Bauraing, A. Deplano, R. Vanhoof, R. De Mendonca, H. Rodriguez-Villalobos, M. Struelens, and Y. Glupczynski
Emergence of ArmA and RmtB aminoglycoside resistance 16S rRNA methylases in Belgium
J. Antimicrob. Chemother., March 1, 2007; 59(3): 459 - 464.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
Y.-J. Park, S. Lee, J. K. Yu, G.-J. Woo, K. Lee, and Y. Arakawa
Co-production of 16S rRNA methylases and extended-spectrum {beta}-lactamases in AmpC-producing Enterobacter cloacae, Citrobacter freundii and Serratia marcescens in Korea
J. Antimicrob. Chemother., October 1, 2006; 58(4): 907 - 908.
[Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
J.-i. Wachino, K. Yamane, K. Kimura, N. Shibata, S. Suzuki, Y. Ike, and Y. Arakawa
Mode of Transposition and Expression of 16S rRNA Methyltransferase Gene rmtC Accompanied by ISEcp1.
Antimicrob. Agents Chemother., September 1, 2006; 50(9): 3212 - 3215.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
M. A. Toleman, P. M. Bennett, and T. R. Walsh
ISCR Elements: Novel Gene-Capturing Systems of the 21st Century?
Microbiol. Mol. Biol. Rev., June 1, 2006; 70(2): 296 - 316.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
J.-i. Wachino, K. Yamane, K. Shibayama, H. Kurokawa, N. Shibata, S. Suzuki, Y. Doi, K. Kimura, Y. Ike, and Y. Arakawa
Novel Plasmid-Mediated 16S rRNA Methylase, RmtC, Found in a Proteus mirabilis Isolate Demonstrating Extraordinary High-Level Resistance against Various Aminoglycosides
Antimicrob. Agents Chemother., January 1, 2006; 50(1): 178 - 184.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
M. Galimand, S. Sabtcheva, P. Courvalin, and T. Lambert
Worldwide Disseminated armA Aminoglycoside Resistance Methylase Gene Is Borne by Composite Transposon Tn1548
Antimicrob. Agents Chemother., July 1, 2005; 49(7): 2949 - 2953.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.