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JAC Advance Access originally published online on October 7, 2004
Journal of Antimicrobial Chemotherapy 2004 54(5):932-935; doi:10.1093/jac/dkh446
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JAC vol.54 no.5 © The British Society for Antimicrobial Chemotherapy 2004; all rights reserved

Identification of a chromosome-borne class C ß-lactamase from Erwinia rhapontici

Thierry Naas*, Daniel Aubert, Sophie Vimont and Patrice Nordmann

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France

* Corresponding author. Tel: +33-1-45-21-29-86; Fax: +33-1-45-21-63-40; Email: thierry.naas{at}bct.ap-hop-paris.fr

Objectives: To characterize the ß-lactamase gene content of Erwinia rhapontici.

Methods: The ß-lactamase gene was cloned, sequenced and expressed in Escherichia coli.

Results: The cloned gene conferred a resistance pattern of an Ambler class C ß-lactamase in E. coli. The AmpC-type enzyme had a pI value of 8.6 and shared 62% amino acid sequence identity with that of Escherichia fergusonii. The ampC gene was associated with a regulatory ampR gene and ß-lactamase production was inducible.

Conclusions: This work provides further evidence of the molecular heterogeneity of ß-lactamases in Erwinia spp. and that plant-pathogenic enterobacterial species may constitute a reservoir of antibiotic resistance genes.

Keywords: AmpC , inducible , cephalosporinases , E. rhapontici


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