Skip Navigation


JAC Advance Access originally published online on July 1, 2004
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
54/2/472    most recent
dkh346v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (6)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Stallmann, H. P.
Right arrow Articles by Wuisman, P. I. J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stallmann, H. P.
Right arrow Articles by Wuisman, P. I. J. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Antimicrobial Chemotherapy 2004 54(2):472-476; doi:10.1093/jac/dkh346
JAC vol.54 no.2 © The British Society for Antimicrobial Chemotherapy 2004; all rights reserved.

Osteomyelitis prevention in rabbits using antimicrobial peptide hLF1-11- or gentamicin-containing calcium phosphate cement

Hein P. Stallmann1,2, Christopher Faber1,2, Antonius L. J. J. Bronckers2, Arie V. Nieuw Amerongen3 and Paul I. J. M. Wuisman1,*

Departments of 1 Orthopaedic Surgery, VU University Medical Center, PO Box 7057, 1007 MB Amsterdam; 2 Oral Cell Biology/ACTA; 3 Oral Biochemistry/ACTA, vd Boechorststraat 7, 1081 BT Amsterdam, The Netherlands

* Corresponding author. Tel: +31-20-4442355; Fax: +31-20-4442357; Email: orthop{at}vumc.nl

Objectives: The efficacy of prophylactic treatment with human lactoferrin 1–11 (hLF1-11), a broad-spectrum antimicrobial peptide, was studied in a rabbit model of femur infection.

Methods: Calcium phosphate cement with 50 mg/g hLF1-11 or gentamicin was injected into the femoral canal, after inoculation with Staphylococcus aureus. Three weeks later, slices of the proximal femora were sawn for quantitative bacterial culture and histology.

Results: Treatment with hLF1-11 (P<0.038) or gentamicin (P<0.008) caused a reduction of cfu compared with the untreated control rabbits. The number of sterile cultures was higher in hLF1-11- (3/7) and gentamicin- (5/6) treated animals than in controls (1/7). Radiological and histological analysis showed early bone ingrowth into the cement cracks, and only moderate pathological changes in rabbits with positive cultures.

Conclusions: Local prophylaxis with hLF1-11 effectively reduced development of osteomyelitis in a rabbit model, but gentamicin resulted in a larger number of sterile femora.

Keywords: biodegradable carriers , drug delivery , intramedullary infections , peptide antibiotics , animal models


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
C. Faber, H. P. Stallmann, D. M. Lyaruu, U. Joosten, C. von Eiff, A. van Nieuw Amerongen, and P. I. J. M. Wuisman
Comparable Efficacies of the Antimicrobial Peptide Human Lactoferrin 1-11 and Gentamicin in a Chronic Methicillin-Resistant Staphylococcus aureus Osteomyelitis Model
Antimicrob. Agents Chemother., June 1, 2005; 49(6): 2438 - 2444.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.