Skip Navigation


JAC Advance Access originally published online on July 14, 2004
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
54/2/348    most recent
dkh367v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (12)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Gould, V. C.
Right arrow Articles by Avison, M. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gould, V. C.
Right arrow Articles by Avison, M. B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Antimicrobial Chemotherapy 2004 54(2):348-353; doi:10.1093/jac/dkh367
JAC vol.54 no.2 © The British Society for Antimicrobial Chemotherapy 2004; all rights reserved.

Analysis of sequence variation among smeDEF multi drug efflux pump genes and flanking DNA from defined 16S rRNA subgroups of clinical Stenotrophomonas maltophilia isolates

Virginia C. Gould, Aki Okazaki, Robin A. Howe and Matthew B. Avison*

Bristol Centre for Antimicrobial Research and Evaluation, Department of Pathology & Microbiology, University of Bristol, School of Medical Sciences, University Walk, Bristol. BS8 1TD. UK

* Corresponding author. Tel: +44-117-9287897; Fax: +44-117-9287896; Email: matthewb.avison{at}bris.ac.uk

Objectives: To determine the level of variation in the smeDEF efflux pump and smeT transcriptional regulator genes among three defined 16S rRNA sequence subgroups of clinical Stenotrophomonas maltophilia isolates.

Methods: smeDEF sequencing used a PCR genome walking approach. Determination of the sequence surrounding smeDEF used a flanking primer PCR method and specific primers anchored in smeD or smeF together with random primers.

Results: smeDEF is chromosomal and located in the same position in the chromosome in all three subgroups of isolates. Flanking smeD is a gene, smeT, encoding a putative transcriptional repressor for smeDEF. Variation at these loci among the isolates is considerably lower (up to 10%) than at intrinsic ß-lactamase loci (up to 30%) in the same isolates, implying greater functional constraint. The smeD–smeT intergenic region contains a highly conserved section, which maps with previously predicted promoter/operator regions, and a hypervariable untranslated region, which can be used to subgroup clinical isolates.

Conclusions: These data provide further evidence that it is possible to group clinical isolates of the inherently variable species, S. maltophilia, based on genotypic properties. Isolate D457, in which most work concerning smeDEF expression has been performed, does not fall into S. maltophilia subgroup A, which is the most typical.

Keywords: multidrug resistance , phylogenetic trees , typing


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
A. Okazaki and M. B. Avison
Induction of L1 and L2 {beta}-Lactamase Production in Stenotrophomonas maltophilia Is Dependent on an AmpR-Type Regulator
Antimicrob. Agents Chemother., April 1, 2008; 52(4): 1525 - 1528.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Pilhofer, A. P. Bauer, M. Schrallhammer, L. Richter, W. Ludwig, K.-H. Schleifer, and G. Petroni
Characterization of bacterial operons consisting of two tubulins and a kinesin-like gene by the novel Two-Step Gene Walking method
Nucleic Acids Res., November 29, 2007; 35(20): e135 - e135.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
V. C. Gould and M. B. Avison
SmeDEF-mediated antimicrobial drug resistance in Stenotrophomonas maltophilia clinical isolates having defined phylogenetic relationships
J. Antimicrob. Chemother., June 1, 2006; 57(6): 1070 - 1076.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
V. C. Gould, A. Okazaki, and M. B. Avison
{beta}-Lactam resistance and {beta}-lactamase expression in clinical Stenotrophomonas maltophilia isolates having defined phylogenetic relationships
J. Antimicrob. Chemother., February 1, 2006; 57(2): 199 - 203.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
V. I. Enne, A. A. Delsol, G. R. Davis, S. L. Hayward, J. M. Roe, and P. M. Bennett
Assessment of the fitness impacts on Escherichia coli of acquisition of antibiotic resistance genes encoded by different types of genetic element
J. Antimicrob. Chemother., September 1, 2005; 56(3): 544 - 551.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.