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JAC Advance Access originally published online on June 9, 2004
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Journal of Antimicrobial Chemotherapy 2004 54(1):263-265; doi:10.1093/jac/dkh291
JAC vol.54 no.1 © The British Society for Antimicrobial Chemotherapy 2004; all rights reserved.

Application of microdialysis to cancellous bone tissue for measurement of gentamicin levels

Lars B. Stolle1,*, Magnus Arpi2, Peter Holmberg-Jørgensen3, Per Riegels-Nielsen4 and Johnny Keller3

1 Institute of Experimental Clinical Research and 2 Department of Clinical Microbiology, Aarhus University Hospital, Skejby Hospital, Brendstrupgaardvej, 8200 Aarhus; 3 Department of Orthopaedic Surgery, Aarhus University Hospital, Aarhus Hospitals, Nørrebrogade 44, 8000 Aarhus C; 4 Department of Orthopaedic Surgery, Esbjerg/Varde Hospital, Østergade 8, 6700 Esbjerg, Denmark

* Corresponding author. Tel: +45-89495511; Fax: +45-89496011; Email: stolle{at}iekf.au.dk

Objectives: Knowledge concerning the distribution of antibiotics in bone tissue is valuable for pharmacokinetic and clinical use. Unfortunately, appropriate techniques are difficult to apply. We introduced microdialysis catheters to cancellous bone tissue for the investigation of gentamicin levels and compared the pharmacokinetics measured with values obtained from bone samples.

Methods: After two microdialysis catheters had been inserted into cancellous bone, eight pigs received an intravenous bolus of 240 mg of gentamicin. Microdialysates and bone samples were obtained over a period of 6 h and drug concentrations were measured.

Results: The area under the curves of the two microdialysates and bone samples were 1569, 1721 and 1533 mg·min/L (ANOVA, P=0.81). Reproducibility of the measurements from the microdialysates was defined as the mean ratio of AUC6/catheter no. 1/AUC6/catheter no. 2. This ratio was 1.02.

Conclusions: Microdialysis is a suitable, relatively non-invasive and reproducible technique for dynamic and quantitative measurement of gentamicin levels in experimental research.

Keywords: experimental research , bioassays , bone samples , pharmacokinetics


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