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JAC Advance Access originally published online on June 9, 2004
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Journal of Antimicrobial Chemotherapy 2004 54(1):130-133; doi:10.1093/jac/dkh320
JAC vol.54 no.1 © The British Society for Antimicrobial Chemotherapy 2004; all rights reserved.

Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis

Dihadenys Lemus1, Anandi Martin2,*, Ernesto Montoro1, Françoise Portaels2 and Juan Carlos Palomino2

1 Instituto de Medicina Tropical ‘Pedro Kouri’, La Habana, Cuba; 2 Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat, 155, Antwerp, B-2000 Belgium

* Corresponding author. Tel: +32-3-2476334; Fax: +32-3-2476333; Email: amartin{at}itg.be

Objective: To study the performance of three rapid low cost methods for the detection of rifampicin resistance.

Methods: A panel of 20 coded Mycobacterium tuberculosis strains was tested blindly by the low cost methods: nitrate reductase, MTT and resazurin assays, and compared with the results obtained with the gold standard methods: the proportion method on Löwenstein–Jensen medium and the BACTEC TB 460 system. We have also tested two commercial tests: MGIT and INNO LiPA Rif.TB kit.

Results: Complete agreement was observed among all methods.

Conclusion: These three simple methods might become inexpensive alternative procedures for rapid detection of rifampicin resistance in low-resource countries.

Keywords: M. tuberculosis , MTT , resazurin , nitrate reductase assay , rifampicin


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