JAC Advance Access originally published online on May 5, 2004
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Journal of Antimicrobial Chemotherapy (2004) 53, 952-957
© 2004 The British Society for Antimicrobial Chemotherapy
Molecular characterization of class 1 integrons from Irish thermophilic Campylobacter spp.
1 Molecular Diagnostics Unit, Cork Institute of Technology, Bishopstown, Cork; 2 Department of Medical Microbiology, Cork University Hospital, Wilton, Cork; 3 Irish Equine Centre, Naas, Co. Kildare; 4 Centre for Food Safety, Faculties of Agriculture, Medicine and Veterinary Medicine, University College, Belfield, Dublin 4, Ireland
Received 17 December 2003; returned 12 January 2004; revised 18 February 2004; accepted 23 February 2004
Objectives: In this study a large random collection (n = 378) of Irish thermophilic Campylobacter isolates were investigated for the presence of integrons, genetic elements associated with the dissemination of antimicrobial resistance.
Methods: Purified genomic DNA from each isolate was analysed by PCR for the presence of class 1 integrons. Four gene cassette-associated amplicons were completely characterized.
Results: Sixty-two of the isolates possessed a complete class 1 integron with a recombined gene cassette located within a 1.0 kb amplicon containing an aadA2 gene. This cassette was present in both Campylobacter jejuni and Campylobacter coli isolates and following sequence analysis was shown to be similar to sequences recently reported in Salmonella enterica Hadar and on an 85 kb plasmid conferring quinolone resistance in Escherichia coli.
Conclusions: Aminoglycoside aadA2-encoding class 1 integrons were identified among unrelated Campylobacter spp. Amino acid sequence comparisons revealed identical structures in both Salmonella and E. coli. The presence of class 1 integrons in Campylobacter spp. may be significant should these organisms enter the food chain and especially when antimicrobial treatment for severe infections is being considered.
Keywords: antimicrobial resistance, Campylobacter, gene cassettes, gene organization
* Present address. Dairy Products Research Centre, Teagasc, Moorepark, Fermoy, Co. Cork, Ireland
Correspondence address. Centre for Food Safety, University College, Belfield, Dublin 4, Ireland. Tel: +353-1-716-6082; Fax: +353-1-716-6091; E-mail: sfanning{at}ucd.ie
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