Skip Navigation


JAC Advance Access originally published online on April 29, 2004
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
53/6/1004    most recent
dkh192v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (18)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Ebner, P.
Right arrow Articles by Mathew, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ebner, P.
Right arrow Articles by Mathew, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


Journal of Antimicrobial Chemotherapy (2004) 53, 1004-1009
© 2004 The British Society for Antimicrobial Chemotherapy

Class 1 integrons in various Salmonella enterica serovars isolated from animals and identification of genomic island SGI1 in Salmonella enterica var. Meleagridis

Paul Ebner*, Kimberly Garner§ and Alan Mathew

Food Safety Center of Excellence, University of Tennessee, 2505 River Drive, Knoxville, TN 37996, USA

Received 15 August 2003; returned 13 November 2003; revised 17 February 2004; accepted 22 February 2004

Objectives: To determine the prevalence of integron-mediated antibiotic resistance in a diverse sample set of Salmonella enterica isolated from animals.

Materials and methods: Multiplex PCR was used to detect class 1 integron gene sequences, and integron gene cassettes were identified by PCR mapping. Susceptibility to 18 antibiotics or antibiotic combinations commonly used in either human or veterinary medicine was measured using a microdilution method, and statistical comparisons of the frequency of resistance between groups were made using Fisher’s two-sided probability test. Genotypic comparisons of isolates were made following pulsed-field gel electrophoresis of genomic DNA.

Results: Thirty-two (30.8%) of 104 isolates contained class 1 integron sequences. Integron-positive isolates represented 15 different S. enterica serovars, were obtained from nine different animal species and had a higher frequency of non-integron-mediated antibiotic resistance (P < 0.05) compared with integron-negative isolates. One non-Typhimurium isolate (S. enterica Meleagridis) contained an SGI1 genomic island, including the antibiotic resistance gene cluster.

Conclusions: These data demonstrate that integron-mediated antibiotic resistance is common among diverse Salmonella serovars, many of them rare. In addition, SGI1 is not limited to Salmonella enterica Typhimurium DT104 or other commonly isolated serovars.

Keywords: antibiotic resistance, integrons, Salmonella, SGI1

* Corresponding author. Present address: Louisiana State University Health Sciences Center, 1501 Kings Highway, PO Box 33932, Shreveport, LA 71130-3932, USA. Tel: +1-318-675-5759; Fax: +1-318-675-5764; E-mail: pebner{at}lsuhsc.edu

§ Present address: Department of Biology, Emory University, 1510 Clifford Road, Rollins Research Room 1166, Atlanta, GA 30322, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
M. Gillings, Y. Boucher, M. Labbate, A. Holmes, S. Krishnan, M. Holley, and H. W. Stokes
The Evolution of Class 1 Integrons and the Rise of Antibiotic Resistance
J. Bacteriol., July 15, 2008; 190(14): 5095 - 5100.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
R. S. Levings, S. P. Djordjevic, and R. M. Hall
SGI2, a Relative of Salmonella Genomic Island SGI1 with an Independent Origin
Antimicrob. Agents Chemother., July 1, 2008; 52(7): 2529 - 2537.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
A. T. T. Vo, E. van Duijkeren, A. C. Fluit, and W. Gaastra
A novel Salmonella genomic island 1 and rare integron types in Salmonella Typhimurium isolates from horses in The Netherlands
J. Antimicrob. Chemother., April 1, 2007; 59(4): 594 - 599.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
A. M. Ahmed, A. I. A. Hussein, and T. Shimamoto
Proteus mirabilis clinical isolate harbouring a new variant of Salmonella genomic island 1 containing the multiple antibiotic resistance region
J. Antimicrob. Chemother., February 1, 2007; 59(2): 184 - 190.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
R. S. Levings, S. R. Partridge, S. P. Djordjevic, and R. M. Hall
SGI1-K, a Variant of the SGI1 Genomic Island Carrying a Mercury Resistance Region, in Salmonella enterica Serovar Kentucky
Antimicrob. Agents Chemother., January 1, 2007; 51(1): 317 - 323.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
P. Antunes, J. Machado, and L. Peixe
Characterization of antimicrobial resistance and class 1 and 2 integrons in Salmonella enterica isolates from different sources in Portugal
J. Antimicrob. Chemother., August 1, 2006; 58(2): 297 - 304.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
R. S. Levings, D. Lightfoot, S. R. Partridge, R. M. Hall, and S. P. Djordjevic
The Genomic Island SGI1, Containing the Multiple Antibiotic Resistance Region of Salmonella enterica Serovar Typhimurium DT104 or Variants of It, Is Widely Distributed in Other S. enterica Serovars
J. Bacteriol., July 1, 2005; 187(13): 4401 - 4409.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
B. Doublet, S. Schwarz, C. Kehrenberg, and A. Cloeckaert
Florfenicol Resistance Gene floR Is Part of a Novel Transposon
Antimicrob. Agents Chemother., May 1, 2005; 49(5): 2106 - 2108.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.