JAC Advance Access originally published online on January 28, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Journal of Antimicrobial Chemotherapy (2004) 53, 451-456
© 2004 The British Society for Antimicrobial Chemotherapy
Pseudomonas aeruginosa strains harbouring an unusual blaVIM-4 gene cassette isolated from hospitalized children in Poland (1998–2001)
ska1
anowska11 The Children’s Memorial Health Institute, Warsaw, Poland; 2 Department of Pathology and Microbiology, The Medical Building, University Walk, The University of Bristol, Bristol BS8 1TD, UK; 3 The Jones Group, JMI Laboratories, North Liberty, Iowa, USA
Received 10 October 2003; returned 4 November 2003; revised 1 December 2003; accepted 1 December 2003
Objectives: During 1997–2001, 151 isolates of imipenem-resistant Pseudomonas aeruginosa were obtained from clinical specimens taken from children hospitalized in Warsaw, Poland. These strains were investigated further to determine the mechanism of resistance.
Methods: The strains were analysed by a combination of genotyping and PCR-based strategies.
Results: Eleven of these strains were found to contain the metallo-ß-lactamase (MßL) gene blaVIM-4. The first strain appeared in 1998, and P. aeruginosa strains harbouring this MßL have become endemic in this hospital since then. All P. aeruginosa strains belonged to serotype O:6, and PFGE analysis revealed four different patterns and three sub-types. All 11 MßL-producing strains contained an identical class 1 integron with the usual 5' and 3' conserved sequences. The integron included two resistance cassettes, aacA4 in the first position and the blaVIM-4 cassette in the second position. The blaVIM-4 gene included an unusual direct repeat of 169 bp of the 3' portion of the blaVIM-4 gene.
Conclusions: An unusual blaVIM-4 MßL has become endemic in P. aeruginosa isolates infecting Polish children hospitalized on surgical wards. The formation of this unusual blaVIM-4 gene cassette could be explained by a mechanism involving deletion of a segment of an ancestral tandem repeat of blaVIM-4 via slipped strand replication, mediated by a combination of polymerase and integrase.
Keywords: metallo-ß-lactamases, serotypes, PFGE, ß-lactams, carbapenems
* Corresponding author. Tel:+44-117-928-7522; E-mail: mark.toleman{at}bris.ac.uk
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Strateva and D. Yordanov Pseudomonas aeruginosa - a phenomenon of bacterial resistance J. Med. Microbiol., September 1, 2009; 58(9): 1133 - 1148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Patzer, T. R. Walsh, J. Weeks, D. Dzierzanowska, and M. A. Toleman Emergence and persistence of integron structures harbouring VIM genes in the Children's Memorial Health Institute, Warsaw, Poland, 1998-2006 J. Antimicrob. Chemother., February 1, 2009; 63(2): 269 - 273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Patzer, D. Dzierzanowska, and P. J. Turner Trends in antimicrobial susceptibility of Gram-negative isolates from a paediatric intensive care unit in Warsaw: results from the MYSTIC programme (1997-2007) J. Antimicrob. Chemother., August 1, 2008; 62(2): 369 - 375. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Loli, L. S. Tzouvelekis, D. Gianneli, E. Tzelepi, and V. Miriagou Outbreak of Acinetobacter baumannii with Chromosomally Encoded VIM-1 Undetectable by Imipenem-EDTA Synergy Tests Antimicrob. Agents Chemother., May 1, 2008; 52(5): 1894 - 1896. [Full Text] [PDF]
|
|
|
|
|
|
P. Bogaerts, T.-D. Huang, H. Rodriguez-Villalobos, C. Bauraing, A. Deplano, M. J. Struelens, and Y. Glupczynski Nosocomial infections caused by multidrug-resistant Pseudomonas putida isolates producing VIM-2 and VIM-4 metallo-{beta}-lactamases J. Antimicrob. Chemother., March 1, 2008; 61(3): 749 - 751. [Full Text] [PDF]
|
|
|
|
|
|
B. Libisch, M. Muzslay, M. Gacs, J. Minarovits, M. Knausz, J. Watine, G. Ternak, E. Kenez, I. Kustos, L. Rokusz, et al. Molecular Epidemiology of VIM-4 Metallo-{beta}-Lactamase-Producing Pseudomonas sp. Isolates in Hungary Antimicrob. Agents Chemother., December 1, 2006; 50(12): 4220 - 4223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. G. Giske, B. Libisch, C. Colinon, E. Scoulica, L. Pagani, M. Fuzi, G. Kronvall, and G. M. Rossolini Establishing Clonal Relationships between VIM-1-Like Metallo-{beta}-Lactamase-Producing Pseudomonas aeruginosa Strains from Four European Countries by Multilocus Sequence Typing J. Clin. Microbiol., December 1, 2006; 44(12): 4309 - 4315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li, L. Shi, W. Yang, L. Li, and S. Yamasaki New Array of aacA4-catB3-dfrA1 Gene Cassettes and a Noncoding Cassette from a Class-1-Integron-Positive Clinical Strain of Pseudomonas aeruginosa. Antimicrob. Agents Chemother., June 1, 2006; 50(6): 2278 - 2279. [Full Text] [PDF]
|
|
|
|
|
|
J. Fiett, A. Baraniak, A. Mrowka, M. Fleischer, Z. Drulis-Kawa, L. Naumiuk, A. Samet, W. Hryniewicz, and M. Gniadkowski Molecular Epidemiology of Acquired-Metallo-{beta}-Lactamase-Producing Bacteria in Poland Antimicrob. Agents Chemother., March 1, 2006; 50(3): 880 - 886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. G. Gacar, K. Midilli, F. Kolayli, K. Ergen, S. Gundes, S. Hosoglu, A. Karadenizli, and H. Vahaboglu Genetic and Enzymatic Properties of Metallo-{beta}-Lactamase VIM-5 from a Clinical Isolate of Enterobacter cloacae Antimicrob. Agents Chemother., October 1, 2005; 49(10): 4400 - 4403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. D. Pitout, D. B. Gregson, L. Poirel, J.-A. McClure, P. Le, and D. L. Church Detection of Pseudomonas aeruginosa Producing Metallo-{beta}-Lactamases in a Large Centralized Laboratory J. Clin. Microbiol., July 1, 2005; 43(7): 3129 - 3135. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. R. Walsh, M. A. Toleman, L. Poirel, and P. Nordmann Metallo-{beta}-Lactamases: the Quiet before the Storm? Clin. Microbiol. Rev., April 1, 2005; 18(2): 306 - 325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Toleman, D. Biedenbach, D. M. C. Bennett, R. N. Jones, and T. R. Walsh Italian metallo-{beta}-lactamases: a national problem? Report from the SENTRY Antimicrobial Surveillance Programme J. Antimicrob. Chemother., January 1, 2005; 55(1): 61 - 70. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Castanheira, M. A. Toleman, R. N. Jones, F. J. Schmidt, and T. R. Walsh Molecular Characterization of a {beta}-Lactamase Gene, blaGIM-1, Encoding a New Subclass of Metallo-{beta}-Lactamase Antimicrob. Agents Chemother., December 1, 2004; 48(12): 4654 - 4661. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Libisch, M. Gacs, K. Csiszar, M. Muzslay, L. Rokusz, and M. Fuzi Isolation of an Integron-Borne blaVIM-4 Type Metallo-{beta}-Lactamase Gene from a Carbapenem-Resistant Pseudomonas aeruginosa Clinical Isolate in Hungary Antimicrob. Agents Chemother., September 1, 2004; 48(9): 3576 - 3578. [Abstract] [Full Text] [PDF]
|
|