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JAC Advance Access originally published online on September 30, 2003
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Journal of Antimicrobial Chemotherapy (2003) 52, 853-855
© 2003 The British Society for Antimicrobial Chemotherapy

Continuous-release or burst-release of the antimicrobial peptide human lactoferrin 1-11 (hLF1-11) from calcium phosphate bone substitutes

Hein P. Stallmann1,2, Christopher Faber1,2, Eveline T. Slotema1, D. M. Lyaruu2, Antonius L. J. J. Bronckers2, Arie V. Nieuw Amerongen3 and Paul I. J. M. Wuisman1,*

Departments of 1 Orthopaedic Surgery/VU University Medical Center, PO Box 7057; 2 Oral Cell Biology/ACTA; 3 Oral Biochemistry/ACTA, 1007 MB Amsterdam, The Netherlands

Received 11 February 2003; returned 6 May 2003; revised 13 August 2003; accepted 13 August 2003

Objectives: In order to identify possible drug delivery systems against resistant bone infection, we determined the release of the antimicrobial peptide (AMP) human lactoferrin 1-11 (hLF1-11) from commercially available bone substitutes.

Methods: We combined six calcium phosphate cements and six granule-types with 5 mg/g hLF1-11 and measured its availability and release in vitro from cements (7 days) and granules (3 days). The integrity and antimicrobial activity of the hLF1-11 that was released during the first 24 h were measured, using mass spectrometry, and a killing assay on methicillin-resistant Staphylococcus aureus (MRSA).

Results: Most of the cements showed burst release followed by low-level continuous release, whereas the coated granules showed high burst release for 24 h. After release the peptide was active (in nine of 12 materials) and intact.

Conclusions: Different release profiles may be obtained by choosing the appropriate carrier, which supports the feasibility of biodegradable carriers releasing AMPs against resistant infections.

Keywords: bone infections, human lactoferrin, biodegradable, carriers

* Corresponding author. Tel: +31-20-4442355; Fax: +31-20-4442357; E-mail: orthop{at}vumc.nl


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