JAC Advance Access originally published online on June 12, 2003
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Journal of Antimicrobial Chemotherapy (2003) 52, 116-119
© 2003 The British Society for Antimicrobial Chemotherapy
Evolution of an integron carrying blaVIM-2 in Eastern Europe: report from the SENTRY Antimicrobial Surveillance Program
1 Department of Pathology and Microbiology, University of Bristol, Bristol BS8 1TD, UK; 2 Central Research Laboratory, Warsaw, Poland; 3 The JONES Group/JMI Laboratories, North Liberty, IA; 4 Tufts University School of Medicine, Boston, MA, USA
Received 22 November 2002; returned 18 December 2002; revised 14 April 2003; accepted 16 April 2003
As part of the SENTRY Antimicrobial Surveillance Program, an imipenem-resistant Pseudomonas aeruginosa strain (81-11963A) was isolated from the blood culture of a female neonate institutionalized at the local children’s hospital in Warsaw, Poland. Cloning of an imipenem resistance determinant revealed it to be a VIM-2 metallo-ß-lactamase, but sequence analysis of DNA adjacent to blaVIM-2 revealed it to have a unique gene context. Downstream of the blaVIM-2 gene resides an aacA4 gene encoding the AAC(6')-Ib aminoglycoside acetyltransferase. The integron containing blaVIM-2 shows high similarity to that reported from In58 in France but was novel in that it possessed a gene cassette with a 59 truncated base element only 19 base pairs (bp) long, consisting of a conserved core site and an inverse core site separated by only 5 bp. This appears to be the first report of a metallo-ß-lactamase gene arising from a pathogenic strain in Eastern Europe.
Keywords: metallo-ß-lactamases, Poland, Pseudomonas aeruginosa
* Correspondence address. Bristol Centre for Antibiotic Research and Evaluation, University of Bristol, Bristol BS8 1TD, UK. Tel: +44-117-928-7897; Fax: +44-117-928-7896; E-mail: t.r.walsh{at}bristol.ac.uk
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