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JAC Advance Access originally published online on May 13, 2003
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Journal of Antimicrobial Chemotherapy (2003) 51, 1351-1358
© 2003 The British Society for Antimicrobial Chemotherapy

Genetic linkage of the penicillinase gene, amp, and blrAB, encoding the regulator of ß-lactamase expression in Aeromonas spp.

Pannika Niumsup*, Alan M. Simm, Kurshid Nurmahomed, Timothy R. Walsh, Peter M. Bennett and Matthew B. Avison§

Bristol Centre for Antimicrobial Research and Evaluation, Department of Pathology and Microbiology, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK

Received 18 November 2002; returned 15 January 2003; revised 3 March 2003; accepted 12 March 2003

Aeromonas hydrophila T429125, a human clinical isolate, possesses three coordinately inducible ß-lactamases encoded by ampH (class D ß-lactamase), cepH (class C ß-lactamase) and imiH (class B ß-lactamase). We report that upstream of ampH there are two genes, blrA and blrB, encoding a putative two-component regulatory system. PCR studies revealed the same blrAB–amp gene arrangement in all Aeromonas spp. isolates tested; namely, Aeromonas veronii bv. sobria, Aeromonas jandaei, Aeromonas mediae, Aeromonas salmonicida and Aeromonas trota. A dominant mutation in the predicted BlrB kinase domain results in ß-lactamase overexpression in A. hydrophila T429125, but in other ß-lactamase-overexpressing mutants blrAB remains intact. Relative to the parent strain, A. hydrophila T429125, ß-lactamase- overexpressing mutants show a clear hierarchy of increased ß-lactamase expression: ImiH > CepH > AmpH. The same hierarchy is seen following ß-lactam challenge of A. hydrophila T429125, and correlates with the number of blr-tag sequences (TTCAC) found upstream of each ß-lactamase gene: ampH (one), cepH (two) and imiH (three).

Keywords: Aeromonas, ß-lactamase, induction, two-component system, Blr, gene regulation

* Correspondence address. Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK. Tel: +44-117-928-7439; Fax: +44-117-928-8274; E-mail: Matthewb.Avison{at}bris.ac.uk

§ Present address. Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanuloke 65000, Thailand.


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