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JAC Advance Access originally published online on April 14, 2003
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Journal of Antimicrobial Chemotherapy (2003) 51, 1293-1296
© 2003 The British Society for Antimicrobial Chemotherapy

Sequence analysis of cfxA2-like ß-lactamases in Prevotella species

Chantal Giraud-Morin1, Isabelle Madinier1,2 and Thierry Fosse1,*

1 Laboratoire de Bactériologie, Hôpital l’Archet 2, Centre Hospitalier Universitaire, 151 route de Saint Antoine Ginestière, 06202 Nice Cedex 3; 2 Laboratoire Surfaces–Interfaces en Odontologie, Faculté de Chirurgie Dentaire, Nice, France

Received 13 August 2002; returned 9 October 2002; revised 19 February 2003; accepted 20 February 2003

Sixty-two strains of oral (32) and non-oral (30) Prevotella producing ß-lactamases were screened for cfxA by PCR, using an intragenic primer pair. All 62 were cfxA/cfxA2 positive. Fourteen of these strains, representing seven pigmented and seven non-pigmented Prevotella species were submitted to further PCR with specific primers that amplified the whole ß-lactamase structural gene (966 bp). After cloning and sequencing, the deduced amino acid sequences were compared with that of Bacteroides vulgatus CfxA ß-lactamase. All 14 sequences possessed the E272K substitution characteristic of CfxA2. CfxA sensu stricto was not observed in the present series. G83D, F/V189L, W193L and D239Y substitutions were observed more than once, without species specificity. This sequence analysis indicates that most oral and non-oral ß-lactamase-producing Prevotella isolates from French patients produce variants of the CfxA enzyme.

Keywords: anaerobes, antibiotic resistance, ß-lactam, Prevotella

* Corresponding author. Tel: +33-4-92-03-62-14; Fax: +33-4-92-03-65-49; E-mail: fosse{at}unice.fr


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