Skip Navigation


JAC Advance Access originally published online on March 13, 2003
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
51/4/1017    most recent
dkg150v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Cruz, A.
Right arrow Articles by Cabral, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cruz, A.
Right arrow Articles by Cabral, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


Journal of Antimicrobial Chemotherapy (2003) 51, 1017-1020
© 2003 The British Society for Antimicrobial Chemotherapy

Isolation, excystation and axenization of Giardia lamblia isolates: in vitro susceptibility to metronidazole and albendazole

Agostinho Cruz1,2,*, M. Isaura Sousa3, Zaida Azeredo2, Emilia Leite1, J. C. Figueiredo de Sousa3 and Miguel Cabral3

1 Escola Superior de Tecnologia da Saúde do Porto, Rua João Oliveira Ramos 87, 4000-294 Porto; 2 Instituto de Ciências Biomédicas de Abel Salazar, 4099-003 Porto; 3 Laboratório de Microbiologia, Faculdade de Farmácia da Universidade do Porto, 4050-047 Porto, Portugal

Received 11 January 2002; returned 15 May 2002; revised 24 September 2002; accepted 13 January 2003

From 53 samples of human faeces containing Giardia lamblia cysts, 18 isolates were successfully excysted in vitro, and cultivated axenically in TYI-S-33 modified medium. The in vitro effects of metronidazole and albendazole on these isolates were evaluated by the trophozoite adherence inhibition method. The IC50 was between 2.4 and 11.5 µM for metronidazole and 0.027 and 0.192 µM for albendazole. These IC50 values were similar to those found for the ATCC 30888 and 30957 reference isolates. All isolates were susceptible to the antiparasitic drugs tested. These results suggest that resistance of G. lamblia to metronidazole and albendazole does not seem to be a significant problem in our population.

Keywords: Giardia, susceptibility assays, metronidazole, albendazole

* Corresponding author. Tel: +351-225-081-300; Fax: +351-226-180-627; E-mail: agostinho-cruz{at}clix.pt


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Antimicrob ChemotherHome page
J. Muller, M. Sterk, A. Hemphill, and N. Muller
Characterization of Giardia lamblia WB C6 clones resistant to nitazoxanide and to metronidazole
J. Antimicrob. Chemother., August 1, 2007; 60(2): 280 - 287.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.