Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (3)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by McCann, S. J.
Right arrow Articles by Keevil, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McCann, S. J.
Right arrow Articles by Keevil, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


Journal of Antimicrobial Chemotherapy (2002) 50, 107-110
© 2002 The British Society for Antimicrobial Chemotherapy

Assay of teicoplanin in serum: comparison of high-performance liquid chromatography and fluorescence polarization immunoassay

Steven J. McCann1,*, Leslie O. White2,§ and Brian Keevil1

1 Department of Clinical Biochemistry, Wythenshawe Hospital, Southmoor Road, Manchester M23 9LT; 2 Department of Medical Microbiology, Southmead Health Services NHS Trust, Bristol BS10 5NB, UK

Received 20 September 2001; returned 13 February 2002; revised 7 March 2002; accepted 25 March 2002

A simple liquid extraction coupled with reverse-phase HPLC and UV detection was shown to correlate well with fluorescence polarization immunoassay (FPIA) on the Abbott TDx analyser for serum teicoplanin analysis, r2 = 0.974, HPLC = 0.908 TDx + 2.324. A Bland–Altman plot showed no significant bias between the results. The HPLC method was linear over the range 10–100 mg/L. The HPLC method showed very good reproducibility, comparable to FPIA. The inter-assay coefficients of variation were 2.76%, 2.29% and 2.65% at 10.3, 51.4 and 77.1 mg/L, respectively, with intra-assay coefficients of variation of 1.86%, 1.97% and 1.17% at 9.13, 50.65 and 75.1 mg/L, respectively. Recoveries were between 99.1 and 101.8% within the analytical range. The HPLC method described is simple, robust, highly reproducible and suited to a clinical laboratory with the appropriate equipment.

* Corresponding author. Tel: +44-161-291-2135; Fax: +44-161-291-2927; E-mail: steven.mccann{at}smuht.nwest.nhs.uk

§ Deceased.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.