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Journal of Antimicrobial Chemotherapy (2002) 49, 1040-1042
© 2002 The British Society for Antimicrobial Chemotherapy


Correspondence

Susceptibility testing of Bacillus species

J. M. Andrews,* and R. Wise

Department of Microbiology, City Hospital NHS Trust, Birmingham, UK

Sir,

Recent events have highlighted the need for diagnostic laboratories to be aware that isolates of Bacillus species require identification. Should the isolate prove to be Bacillus anthracis, the guidelines issued by the PHLS–CDSC1 suggest that organisms should be tested for antibiotic susceptibility as soon as possible, although no specific recommendations are given.

In this study, conventional agar incorporation MICs2 for Bacillus spp. were determined on Iso-Sensitest agar (ISA) (Oxoid, Basingstoke, UK) and Mueller–Hinton agar (MH) (Oxoid) at an inoculum of 104 cfu/spot, and by a commercial gradient method (Etest; Cambridge Diagnostics, . . . [Full Text of this Article]

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