Journal of Antimicrobial Chemotherapy (2002) 49, 837-840
© 2002 The British Society for Antimicrobial Chemotherapy
Molecular characterization of metallo-b-lactamase-producing Acinetobacter baumannii and Acinetobacter genomospecies 3 from Korea: identification of two new integrons carrying the blaVIM-2 gene cassettes
1Department of Clinical Pathology, Research Institute of Bacterial Resistance, and Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, C.P.O. Box 8044, Seoul, Korea; 2Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, I-53100 Siena, Italy
Received 7 September 2001; returned 9 November 2001; revised 11 December 2001; accepted 28 February 2002.
Carbapenem-resistant Acinetobacter spp. used to be rare, but are increasingly isolated in Korea. Among 28 isolates of imipenem-resistant Acinetobacter spp. found in a Korean hospital in 1998 and 1999, 14 produced metallo-b-lactamases. The blaVIM-2 gene was detected, by PCR, in 11 and two isolates of Acinetobacter baumannii and Acinetobacter genomospecies 3, respectively, and blaIMP-1 in one isolate of A. baumannii. The MICs of imipenem for the isolates were 8–32 mg/L. PFGE analysis of SmaI-digested genomic DNA gave identical patterns in eight of 11 blaVIM-2-positive A. baumannii isolates from respiratory specimens of ICU patients. The blaVIM-2 gene cassettes in the isolates are identical to those from Pseudomonas aeruginosa isolates in Europe, but are inserted into new class I integrons In105 and In106. The attC site of the last cassette of the array in In106 is interrupted by the insertion of a putative class II intron. This is the first report of VIM-2 b-lactamase-producing A. baumannii and Acinetobacter genomospecies 3. Production of the VIM-2 enzyme presents an emerging threat of carbapenem resistance among Acinetobacter spp. in Korea.
* Correspondence address. Department of Clinical Pathology, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752, Korea. Tel: +82-2-361-5866; Fax: +82-2-313-0956; E-mail: leekcp{at}yumc.yonsei.ac.kr
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  G. Leon and P. H. Roy Potential Role of Group IIC-attC Introns in Integron Cassette Formation J. Bacteriol., October 1, 2009; 191(19): 6040 - 6051. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Uma Karthika, R. Srinivasa Rao, S. Sahoo, P. Shashikala, R. Kanungo, S. Jayachandran, and K. Prashanth Phenotypic and genotypic assays for detecting the prevalence of metallo-{beta}-lactamases in clinical isolates of Acinetobacter baumannii from a South Indian tertiary care hospital J. Med. Microbiol., April 1, 2009; 58(4): 430 - 435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Beceiro, A. Perez, F. Fernandez-Cuenca, L. Martinez-Martinez, A. Pascual, J. Vila, J. Rodriguez-Bano, J. M. Cisneros, J. Pachon, G. Bou, et al. Genetic Variability among ampC Genes from Acinetobacter Genomic Species 3 Antimicrob. Agents Chemother., March 1, 2009; 53(3): 1177 - 1184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Marti, J. Sanchez-Cespedes, M. D. Blasco, M. Ruiz, P. Espinal, V. Alba, F. Fernandez-Cuenca, A. Pascual, and J. Vila Characterization of the Carbapenem-Hydrolyzing Oxacillinase Oxa-58 in an Acinetobacter Genospecies 3 Clinical Isolate Antimicrob. Agents Chemother., August 1, 2008; 52(8): 2955 - 2958. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Y. Peleg, H. Seifert, and D. L. Paterson Acinetobacter baumannii: Emergence of a Successful Pathogen Clin. Microbiol. Rev., July 1, 2008; 21(3): 538 - 582. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Tokatlidou, M. Tsivitanidou, S. Pournaras, A. Ikonomidis, A. Tsakris, and D. Sofianou Outbreak Caused by a Multidrug-Resistant Klebsiella pneumoniae Clone Carrying blaVIM-12 in a University Hospital J. Clin. Microbiol., March 1, 2008; 46(3): 1005 - 1008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Perez, A. M. Hujer, K. M. Hujer, B. K. Decker, P. N. Rather, and R. A. Bonomo Global Challenge of Multidrug-Resistant Acinetobacter baumannii Antimicrob. Agents Chemother., October 1, 2007; 51(10): 3471 - 3484. [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Lee, C. H. Choi, H. Y. Kang, J. Y. Lee, J. Kim, Y. C. Lee, S. Y. Seol, D. T. Cho, K. W. Kim, D. Y. Song, et al. Differences in phenotypic and genotypic traits against antimicrobial agents between Acinetobacter baumannii and Acinetobacter genomic species 13TU J. Antimicrob. Chemother., April 1, 2007; 59(4): 633 - 639. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. M. Lim, K. S. Shin, and J. Kim Distinct Antimicrobial Resistance Patterns and Antimicrobial Resistance-Harboring Genes According to Genomic Species of Acinetobacter Isolates J. Clin. Microbiol., March 1, 2007; 45(3): 902 - 905. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Mendes, K. A. Kiyota, J. Monteiro, M. Castanheira, S. S. Andrade, A. C. Gales, A. C. C. Pignatari, and S. Tufik Rapid Detection and Identification of Metallo-{beta}-Lactamase-Encoding Genes by Multiplex Real-Time PCR Assay and Melt Curve Analysis J. Clin. Microbiol., February 1, 2007; 45(2): 544 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Galani, M. Souli, E. Koratzanis, Z. Chryssouli, and H. Giamarellou Molecular characterization of an Escherichia coli clinical isolate that produces both metallo-{beta}-lactamase VIM-2 and extended-spectrum {beta}-lactamase GES-7: identification of the In8 integron carrying the blaVIM-2 gene J. Antimicrob. Chemother., August 1, 2006; 58(2): 432 - 433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Lee, J. H. Yum, D. Yong, H. M. Lee, H. D. Kim, J.-D. Docquier, G. M. Rossolini, and Y. Chong Novel Acquired Metallo-{beta}-Lactamase Gene, blaSIM-1, in a Class 1 Integron from Acinetobacter baumannii Clinical Isolates from Korea Antimicrob. Agents Chemother., November 1, 2005; 49(11): 4485 - 4491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-Z. Su, J. Chen, T. Mizushima, T. Kuroda, and T. Tsuchiya AbeM, an H+-Coupled Acinetobacter baumannii Multidrug Efflux Pump Belonging to the MATE Family of Transporters Antimicrob. Agents Chemother., October 1, 2005; 49(10): 4362 - 4364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kehrenberg, B. Catry, F. Haesebrouck, A. de Kruif, and S. Schwarz Novel Spectinomycin/Streptomycin Resistance Gene, aadA14, from Pasteurella multocida Antimicrob. Agents Chemother., July 1, 2005; 49(7): 3046 - 3049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sunde Class I Integron with a Group II Intron Detected in an Escherichia coli Strain from a Free-Range Reindeer Antimicrob. Agents Chemother., June 1, 2005; 49(6): 2512 - 2514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Poirel, S. Marque, C. Heritier, C. Segonds, G. Chabanon, and P. Nordmann OXA-58, a Novel Class D {beta}-Lactamase Involved in Resistance to Carbapenems in Acinetobacter baumannii Antimicrob. Agents Chemother., January 1, 2005; 49(1): 202 - 208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Swenson, G. E. Killgore, and F. C. Tenover Antimicrobial Susceptibility Testing of Acinetobacter spp. by NCCLS Broth Microdilution and Disk Diffusion Methods J. Clin. Microbiol., November 1, 2004; 42(11): 5102 - 5108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Nagano, Y. Nagano, C. Cordevant, N. Shibata, and Y. Arakawa Nosocomial Transmission of CTX-M-2 {beta}-Lactamase-Producing Acinetobacter baumannii in a Neurosurgery Ward J. Clin. Microbiol., September 1, 2004; 42(9): 3978 - 3984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-S. Yu, Q. Yang, X.-W. Xu, H.-S. Kong, G.-Y. Xu, and B.-Y. Zhong Typing and characterization of carbapenem-resistant Acinetobacter calcoaceticus-baumannii complex in a Chinese hospital J. Med. Microbiol., July 1, 2004; 53(7): 653 - 656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Beceiro, L. Dominguez, A. Ribera, J. Vila, F. Molina, R. Villanueva, J. M. Eiros, and G. Bou Molecular Characterization of the Gene Encoding a New AmpC {beta}-Lactamase in a Clinical Strain of Acinetobacter Genomic Species 3 Antimicrob. Agents Chemother., April 1, 2004; 48(4): 1374 - 1378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Luzzaro, J.-D. Docquier, C. Colinon, A. Endimiani, G. Lombardi, G. Amicosante, G. M. Rossolini, and A. Toniolo Emergence in Klebsiella pneumoniae and Enterobacter cloacae Clinical Isolates of the VIM-4 Metallo-{beta}-Lactamase Encoded by a Conjugative Plasmid Antimicrob. Agents Chemother., February 1, 2004; 48(2): 648 - 650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Toleman, K. Rolston, R. N. Jones, and T. R. Walsh blaVIM-7, an Evolutionarily Distinct Metallo-{beta}-Lactamase Gene in a Pseudomonas aeruginosa Isolate from the United States Antimicrob. Agents Chemother., January 1, 2004; 48(1): 329 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ribera, J. Vila, F. Fernandez-Cuenca, L. Martinez-Martinez, A. Pascual, A. Beceiro, G. Bou, J. M. Cisneros, J. Pachon, and J. Rodriguez-Bano Type 1 Integrons in Epidemiologically Unrelated Acinetobacter baumannii Isolates Collected at Spanish Hospitals Antimicrob. Agents Chemother., January 1, 2004; 48(1): 364 - 365. [Full Text] [PDF]
|
|
|
|
|
|
N. Shibata, Y. Doi, K. Yamane, T. Yagi, H. Kurokawa, K. Shibayama, H. Kato, K. Kai, and Y. Arakawa PCR Typing of Genetic Determinants for Metallo-{beta}-Lactamases and Integrases Carried by Gram-Negative Bacteria Isolated in Japan, with Focus on the Class 3 Integron J. Clin. Microbiol., December 1, 2003; 41(12): 5407 - 5413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-D. Docquier, M. L. Riccio, C. Mugnaioli, F. Luzzaro, A. Endimiani, A. Toniolo, G. Amicosante, and G. M. Rossolini IMP-12, a New Plasmid-Encoded Metallo-{beta}-Lactamase from a Pseudomonas putida Clinical Isolate Antimicrob. Agents Chemother., May 1, 2003; 47(5): 1522 - 1528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Riccio, J.-D. Docquier, E. Dell'Amico, F. Luzzaro, G. Amicosante, and G. M. Rossolini Novel 3-N-Aminoglycoside Acetyltransferase Gene, aac(3)-Ic, from a Pseudomonas aeruginosa Integron Antimicrob. Agents Chemother., May 1, 2003; 47(5): 1746 - 1748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Heritier, L. Poirel, D. Aubert, and P. Nordmann Genetic and Functional Analysis of the Chromosome-Encoded Carbapenem-Hydrolyzing Oxacillinase OXA-40 of Acinetobacter baumannii Antimicrob. Agents Chemother., January 1, 2003; 47(1): 268 - 273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Miriagou, E. Tzelepi, D. Gianneli, and L. S. Tzouvelekis Escherichia coli with a Self-Transferable, Multiresistant Plasmid Coding for Metallo-{beta}-Lactamase VIM-1 Antimicrob. Agents Chemother., January 1, 2003; 47(1): 395 - 397. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Lopez-Otsoa, L. Gallego, K. J. Towner, L. Tysall, N. Woodford, and D. M. Livermore Endemic Carbapenem Resistance Associated with OXA-40 Carbapenemase among Acinetobacter baumannii Isolates from a Hospital in Northern Spain J. Clin. Microbiol., December 1, 2002; 40(12): 4741 - 4743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Yong, K. Lee, J. H. Yum, H. B. Shin, G. M. Rossolini, and Y. Chong Imipenem-EDTA Disk Method for Differentiation of Metallo-{beta}-Lactamase-Producing Clinical Isolates of Pseudomonas spp. and Acinetobacter spp. J. Clin. Microbiol., October 1, 2002; 40(10): 3798 - 3801. [Abstract] [Full Text] [PDF]
|
|