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Journal of Antimicrobial Chemotherapy (2002) 49, 675-678
© 2002 The British Society for Antimicrobial Chemotherapy

Brief report

Production of the RdxA protein in metronidazole-susceptible and -resistant isolates of Helicobacter pylori cultured from treated mice

Stephanie R. Lathama, Agnès Labigneb and Peter J. Jenksa,,c,*

a Department of Medical Microbiology, Royal Free and University College Medical School, Rowland Hill Street, London, UK; b Unité de Pathogénie Bactérienne des Muqueuses, Institut Pasteur, 28 Rue du Dr Roux, Paris, France; c Institute of Infections and Immunity, University of Nottingham, Nottingham NG7 2UH, UK

The objective of this study was to use immunoblotting with RdxA antisera to examine the production of the RdxA protein in mouse-derived metronidazole-susceptible and -resistant isolates of Helicobacter pylori. A 24 kDa immunoreactive band corresponding to RdxA was observed in all 15 metronidazole-susceptible and five of 50 metronidazole-resistant isolates. The rdxA gene of these five isolates contained missense mutations and transformation experiments confirmed that these mutations were associated with inactivation of the rdxA gene. No RdxA protein was produced in the other 45 metronidazole-resistant strains, including one in which the nucleotide sequence of the rdxA gene was unchanged. These results demonstrate a high correlation between production of the RdxA protein and susceptibility of H. pylori to metronidazole. Testing for the absence of the RdxA protein identifies the majority of strains that will respond poorly to metronidazole-containing eradication regimens.

* Correspondence address. Institute of Infections and Immunity, Floor C, West Block, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, UK. Tel: +44-115-924-9924 ext. 42457; Fax: +44-115-970-9923; E-mail: Peter.Jenks{at}nottingham.ac.uk


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