Journal of Antimicrobial Chemotherapy (2002) 49, 269-273
© 2002 The British Society for Antimicrobial Chemotherapy
Characterization of blaCMY-11, an AmpC-type plasmid-mediated ß-lactamase gene in a Korean clinical isolate of Escherichia coli
a Department of Genetic Engineering, Youngdong University, Chungbuk 370-701; b Department of Microbiology, College of Natural Sciences, Research Centre for Molecular Microbiology, Seoul National University, Seoul; c Department of Clinical Pathology, Kosin University College of Medicine, Pusan, and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea
We report the description of a new plasmid-encoded AmpC-type ß-lactamase gene (blaCMY-11) from Escherichia coli K983802.1 that was isolated from a patient in South Korea suffering from a urinary tract infection. Antibiotic susceptibility testing, plasmid analysis, pI determination, transconjugation and Southern blot analysis were carried out to investigate the resistance mechanism to cefoxitin. PCR, sequencing and sequence analysis were used to identify and analyse the ß-lactamase gene (blaCMY-11) responsible for the cefoxitin resistance. CMY-11 and blaCMY-11 are compared with other class C ß-lactamases and their genes to determine phylogenetic relationships. The cefoxitin-resistance phenotype of E. coli K983802.1 reflects the presence of a large plasmid [pYMG-2 (130 kb)]. A ß-lactamase with a pI value of 8.0 from a transconjugant of E. coli K983802.1 was identified by isoelectric focusing. A 1478 bp DNA fragment from pYMG-2 containing blaCMY-11 was sequenced and an open reading frame coding for a 382 amino acid peptide (CMY-11) was found. Phylogenetic analysis clearly shows that blaCMY-11 belongs to the group of ampC-related bla genes. It is likely that blaCMY-11 evolved from blaCMY-1 via blaCMY-10.
* Corresponding author. Tel: +82-43-740-1112; Fax: +82-43-740-1109; E-mail: sanghee{at}youngdong.ac.kr
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