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Journal of Antimicrobial Chemotherapy (2001) 47, 745-754
© 2001 The British Society for Antimicrobial Chemotherapy

Characterization, cloning and sequence analysis of the inducible Ochrobactrum anthropi AmpC ß-lactamase

Catherine S. Higgins, Matthew B. Avison*,, Lee Jamieson, Alan M. Simm, Peter M. Bennett and Timothy R. Walsh

Bristol Centre for Antimicrobial Research and Evaluation, Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK

Ochrobactrum anthropi is resistant to most cephalosporins and penicillins due, at least in part, to the inducible expression of a single ß-lactamase. The ß-lactamase gene has been cloned and sequenced. It encodes an AmpC-type class 1 serine active-site enzyme that hydrolyses mainly cephalosporins and is resistant to inhibition by clavulanic acid. Expression of the ampC gene is inducible via a typical AmpR regulator, which is encoded upstream of ampC. Inducible expression is retained following cloning of O. anthropi ampR–ampC into Escherichia coli, confirming that the signal for AmpR activation in O. anthropi is the same as that used in the Enterobacteriaceae. This is the first reported example of an AmpC ß-lactamase outside of the {gamma}-subdivision of the bacterial kingdom. Genomic searches of other non-{gamma}-subdivision bacteria revealed a homologous ampR–ampC cluster in the plant symbiont, Sinorhizobium meliloti.

* Correspondence address. Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, UK. Tel: +44-117-9287439; Fax: +44-117-9288274; E-mail: Matthewb.Avison{at}bris.ac.uk


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