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Journal of Antimicrobial Chemotherapy (2001) 47, 715-718
© 2001 The British Society for Antimicrobial Chemotherapy

Brief report

Effect of inoculum form on in vitro antifungal susceptibilities of Aspergillus spp.

Isabel Pujola, Joan Fernández-Ballartb and Josep Guarroc,d,*

a Laboratori de Microbiologia, Hospital Universitari de Sant Joan de Reus, Reus, Tarragona; b Unitat de Medicina Preventiva, Facultat de Medicina, Universitat Rovira i Virgili, Reus, Tarragona; c Unitat de Microbiologia, Facultat de Medicina, Universitat Rovira i Virgili, Carrer Sant Llorenç 21, Reus, Tarragona; d Institut d'Estudis Avançats, Universitat Rovira i Virgili, Tarragona, Spain

The effect of inoculum form, i.e. ungerminated conidia, germinated conidia and hyphae on the in vitro antifungal susceptibility of 12 strains belonging to Aspergillus spp. was compared using a broth microdilution method. The isolates were tested three times on different days against amphotericin B, itraconazole and ketoconazole. There were no significant differences between MICs obtained with the three types of inoculum (P > 0.05) for any antifungal tested. The degree of reproducibility of the tests was high (>=75%) for all antifungals with each type of inoculum, except for itraconazole (58.3%) when the inoculum was prepared with ungerminated conidia.

* Correspondence address. Unitat de Microbiologia, Facultat de Medicina, Universitat Rovira i Virgili, Carrer Sant Llorenç 21, 43201 Reus, Tarragona, Spain. Tel: +34-977-759359; Fax: +34-977-759322; E-mail: umb{at}astor.urv.es


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