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Journal of Antimicrobial Chemotherapy (2001) 47, 496-498
© 2001 The British Society for Antimicrobial Chemotherapy

Correspondence

Gentamicin diffusion in Mueller–Hinton agar plates from different manufacturers

B. Comuzzia, C. Arcellonia,*, R. Vaianib and R. Paronia

a Laboratory of Separative Techniques and b Microbiology Service, Department of Laboratory Medicine, Scientific Institute H. S. Raffaele and School of Medicine, Via Olgettina 60, 20132 Milan, Italy

Sir,

The diameter of inhibition zones in agar diffusion susceptibility test is the result of several variables related to the antibiotic–bacterium pair, standard method and composition of the medium.1 The evaluation of different lots of Mueller–Hinton agar (MHA) and the development and application of reference media in the agar diffusion test has been taken into consideration by the NCCLS2 and European microbiological societies.3

The quality controls made by the media manufacturers are based on physical tests of agar matrix composition and on bioassays using organisms with known characteristics: the produced media must be in the range of the . . . [Full Text of this Article]

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