Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (30)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Antignac, A.
Right arrow Articles by Taha, M.-K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Antignac, A.
Right arrow Articles by Taha, M.-K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Antimicrobial Chemotherapy (2001) 47, 285-296
© 2001 The British Society for Antimicrobial Chemotherapy

Polymorphism of Neisseria meningitidis penA gene associated with reduced susceptibility to penicillin

Aude Antignaca, Paula Krizb, Georgina Tzanakakic, Jean-Michel Alonsoa and Muhamed-Kheir Tahaa,*

a Unité des Neisseria and Centre National de Référence des Méningocoques, Institut Pasteur, 28 Rue du Dr Roux, 75724 Paris cedex 15, France; b National Reference Laboratory for Meningococcal Infections, NIPH, Prague, Czech Republic; c National Meningococcal Reference Laboratory, 196 Alexandras Avenue, 11521 Athens, Greece

We studied polymorphism of penA (which encodes penicillin-binding protein 2) in 13 strains of Neisseria meningitidis susceptible to penicillin (penS) and 12 strains with reduced susceptibility to penicillin (penI). These strains differed in geographical origin. Serological and genetic typing showed that they were highly diverse and belonged to several genetic lineages. Restriction analysis and DNA sequencing of penA showed that all penS strains had the same penA allele regardless of genetic group, whereas penI strains harboured various penA alleles. Transformation with amplicons of penA and genomic DNA from several penI strains conferred the penI phenotype on a penS strain. Thus, reduction in susceptibility to penicillin is directly related to changes in penA and analysis of penA polymorphisms could be used as a reliable tool for characterizing meningococcal strains in terms of their susceptibility to penicillin.

* Corresponding author. Tel: +33-1-45688438; Fax: +33-1-45688338; E-mail: mktaha{at}pasteur.fr


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
S. T. Hedberg, H. Fredlund, P. Nicolas, D. A. Caugant, P. Olcen, and M. Unemo
Antibiotic Susceptibility and Characteristics of Neisseria meningitidis Isolates from the African Meningitis Belt, 2000 to 2006: Phenotypic and Genotypic Perspectives
Antimicrob. Agents Chemother., April 1, 2009; 53(4): 1561 - 1566.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
M. du Plessis, A. von Gottberg, C. Cohen, L. de Gouveia, K. P. Klugman, and for the Group for Enteric Respiratory and Meningea
Neisseria meningitidis Intermediately Resistant to Penicillin and Causing Invasive Disease in South Africa in 2001 to 2005
J. Clin. Microbiol., October 1, 2008; 46(10): 3208 - 3214.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
M.-K. Taha, J. A. Vazquez, E. Hong, D. E. Bennett, S. Bertrand, S. Bukovski, M. T. Cafferkey, F. Carion, J. J. Christensen, M. Diggle, et al.
Target Gene Sequencing To Characterize the Penicillin G Susceptibility of Neisseria meningitidis
Antimicrob. Agents Chemother., August 1, 2007; 51(8): 2784 - 2792.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
S. Thulin, P. Olcen, H. Fredlund, and M. Unemo
Total Variation in the penA Gene of Neisseria meningitidis: Correlation between Susceptibility to {beta}-Lactam Antibiotics and penA Gene Heterogeneity.
Antimicrob. Agents Chemother., October 1, 2006; 50(10): 3317 - 3324.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
J. H. Jorgensen, S. A. Crawford, L. C. Fulcher, A. Glennen, S. M. Harrington, J. Swenson, R. Lynfield, P. R. Murray, and F. C. Tenover
Multilaboratory Evaluation of Disk Diffusion Antimicrobial Susceptibility Testing of Neisseria meningitidis Isolates.
J. Clin. Microbiol., May 1, 2006; 44(5): 1744 - 1754.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
M. L. Zarantonelli, A. Antignac, M. Lancellotti, A. Guiyoule, J.-M. Alonso, and M.-K. Taha
Immunogenicity of meningococcal PBP2 during natural infection and protective activity of anti-PBP2 antibodies against meningococcal bacteraemia in mice
J. Antimicrob. Chemother., May 1, 2006; 57(5): 924 - 930.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
M.-K. Taha, M. L. Zarantonelli, A. Neri, R. Enriquez, J. A. Vazquez, and P. Stefanelli
Interlaboratory Comparison of PCR-Based Methods for Detection of Penicillin G Susceptibility in Neisseria meningitidis
Antimicrob. Agents Chemother., March 1, 2006; 50(3): 887 - 892.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
J. H. Jorgensen, S. A. Crawford, and K. R. Fiebelkorn
Susceptibility of Neisseria meningitidis to 16 Antimicrobial Agents and Characterization of Resistance Mechanisms Affecting Some Agents
J. Clin. Microbiol., July 1, 2005; 43(7): 3162 - 3171.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
T. R. Shultz, P. A. White, and J. W. Tapsall
In Vitro Assessment of the Further Potential for Development of Fluoroquinolone Resistance in Neisseria meningitidis
Antimicrob. Agents Chemother., May 1, 2005; 49(5): 1753 - 1760.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
K. R. Fiebelkorn, S. A. Crawford, and J. H. Jorgensen
Mutations in folP Associated with Elevated Sulfonamide MICs for Neisseria meningitidis Clinical Isolates from Five Continents
Antimicrob. Agents Chemother., February 1, 2005; 49(2): 536 - 540.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
P. Stefanelli, C. Fazio, A. Neri, T. Sofia, and P. Mastrantonio
Emergence in Italy of a Neisseria meningitidis Clone with Decreased Susceptibility to Penicillin
Antimicrob. Agents Chemother., August 1, 2004; 48(8): 3103 - 3106.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
P. Stefanelli, A. Carattoli, A. Neri, C. Fazio, and P. Mastrantonio
Prediction of Decreased Susceptibility to Penicillin of Neisseria meningitidis Strains by Real-Time PCR
J. Clin. Microbiol., October 1, 2003; 41(10): 4666 - 4670.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Antignac, J.-C. Rousselle, A. Namane, A. Labigne, M.-K. Taha, and I. G. Boneca
Detailed Structural Analysis of the Peptidoglycan of the Human Pathogen Neisseria meningitidis
J. Biol. Chem., August 22, 2003; 278(34): 31521 - 31528.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Antignac, I. G. Boneca, J.-C. Rousselle, A. Namane, J.-P. Carlier, J. A. Vazquez, A. Fox, J.-M. Alonso, and M.-K. Taha
Correlation between Alterations of the Penicillin-binding Protein 2 and Modifications of the Peptidoglycan Structure in Neisseria meningitidis with Reduced Susceptibility to Penicillin G
J. Biol. Chem., August 22, 2003; 278(34): 31529 - 31535.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
S. Ameyama, S. Onodera, M. Takahata, S. Minami, N. Maki, K. Endo, H. Goto, H. Suzuki, and Y. Oishi
Mosaic-Like Structure of Penicillin-Binding Protein 2 Gene (penA) in Clinical Isolates of Neisseria gonorrhoeae with Reduced Susceptibility to Cefixime
Antimicrob. Agents Chemother., December 1, 2002; 46(12): 3744 - 3749.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
C. L. TROTTER, A. J. FOX, M. E. RAMSAY, F. SADLER, S. J. GRAY, R. MALLARD, and E. B KACZMARSKI
Fatal outcome from meningococcal disease - an association with meningococcal phenotype but not with reduced susceptibility to benzylpenicillin
J. Med. Microbiol., October 1, 2002; 51(10): 855 - 860.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
A. Antignac, J.-M. Alonso, and M.-K. Taha
Nonculture Prediction of Neisseria meningitidis Susceptibility to Penicillin
Antimicrob. Agents Chemother., December 1, 2001; 45(12): 3625 - 3628.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.