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Journal of Antimicrobial Chemotherapy (2000) 46, 713-716
© 2000 The British Society for Antimicrobial Chemotherapy

Influence of different medium components on the in vitro activity of the growth-promoting antibiotic flavomycin against enterococci

Patrick Butaye*, Luc A. Devriese and Freddy Haesebrouck

Laboratory of Veterinary Bacteriology and Mycology, Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, University of Ghent, Salisburylaan 133, B-9820 Merelbeke, Belgium

The growth-promoting antibiotic flavomycin (also called bambermycin, flavophospholipol and moenomycin) has a complex spectrum of activity against enterococci, with some species being naturally resistant and others susceptible. In this study, proteins added to Mueller–Hinton II medium had a strong deleterious effect on the activity of flavomycin, glucose had no effect and starch decreased the activity of flavomycin. The fatty substances Tween 80 and tributyrin increased the activity of flavomycin for several enterococcal species. Slight differences in the composition of the susceptibility test medium affected the MIC results obtained, indicating that strict standardization of the test medium is necessary.

* Corresponding author. Tel: +32-9-264-74-35; Fax: +32-9-264-74-94; E-mail: pbutaye{at}allserv.rug.ac.be


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