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Journal of Antimicrobial Chemotherapy (2000) 46, 87-89
© 2000 The British Society for Antimicrobial Chemotherapy

Brief reports

Two new gene cassettes, dfr17 (for trimethoprim resistance) and aadA4 (for spectinomycin/streptomycin resistance), inserted in an Escherichia coli class 1 integron

Chung-Yu Chang, Lin-Li Chang, Yu-Hung Chang, Tsong-Ming Lee{dagger}, Yuan-Hung Li and Shui-Feng Chang*

Department of Microbiology, School of Medicine, Kaohsiung Medical University, 100, Shih-Chuan 1st Road, Kaohsiung 807, Taiwan, Republic of China

Two new gene cassettes, dfr17 and aadA4, inserted in a class 1 integron of Escherichia coli EC107, are described here. The dfr17 cassette encodes trimethoprim resistance and has 91% identity with the dfrVII dihydrofolate reductase gene. The aadA4 cassette confers resistance to spectinomycin and streptomycin and shows 94% identity with the aadA3 gene. The integron carrying the dfr17 and aadA4 cassettes was located on a conjugative plasmid, pEC1072.

* Corresponding author. Tel: +886-(7)-312-1101, ext. 2145; Fax: +886-(7)-321-8309; E-mail: shfech{at}cc.kmu.edu.tw

{dagger} Present address. Department of Food Sanitation, Tajen Institute of Technology, Ping Tung 907, Taiwan, Republic of China.


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