Journal of Antimicrobial Chemotherapy (2000) 45, 881-885
© 2000 The British Society for Antimicrobial Chemotherapy
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Detection of extended-spectrum ß-lactamases in members of the family Enterobacteriaceae: comparison of the MAST DD test, the double disc and the Etest ESBL
Division of Microbiology, University of Leeds, Leeds LS2 9JT, UK
A technically simple method—the MAST double disc (MDD) test—for the detection of extended-spectrum ß-lactamase (ESBL) production by bacteria is described. A wide range of ESBL, non-ESBL and Class 1 ß-lactamase-producing isolates was examined. The MDD test, which uses discs containing ceftazidime and a complementary disc containing ceftazidime and clavulanate and a second pair containing cefotaxime and cefotaxime and clavulanate was compared with the standard double disc diffusion test and an Etest method. Both the Etest and the MDD correctly identified 93% of ESBL producers. The MDD is an inexpensive alternative to current methods for the detection of ESBL production.
* Corresponding author. Tel: +44-113-233-5597; Fax: +44-113-233-5649; E-mail: michfm{at}leeds.ac.uk
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