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Journal of Antimicrobial Chemotherapy (2000) 45, 139-144
© 2000 The British Society for Antimicrobial Chemotherapy

MecI represses synthesis from the ß-lactamase operon of Staphylococcus aureus

Richard A. Lewis{dagger} and Keith G. H. Dyke*

University of Oxford, Microbiology Unit, Department of Biochemistry, South Parks Road, Oxford OX1 3QU, UK

Plasmid diploids were constructed in Staphylococcus aureus to study the effect of the repressor of methicillin resistance (MecI) on the synthesis of both ß-lactamase and the ß-lactamase repressor (BlaI). MecI-mediated repression of the synthesis of ß-lactamase was shown by reduction in the specific activity of nitrocefinase in bacteria containing a plasmid carrying mecI but not when containing the same plasmid deleted for mecI. Antisera prepared against purified MecI and against purified BlaI were used in Western blots which showed that MecI repressed the synthesis of BlaI in these diploids. The interactions between the mec operon and the bla operon are discussed.

{dagger} Present address. School of Biological Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.

* Corresponding author. Tel: +44-1865-275293; Fax: +44-1865-275297; E-mail: kdyke{at}bioch.ox.ac.uk


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