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Journal of Antimicrobial Chemotherapy (1999) 44, 831-834
© 1999 The British Society for Antimicrobial Chemotherapy

Brief report

Modified time–kill assay against multidrug-resistant Enterococcus faecium with novel antimicrobial combinations

Chad R. Messicka, Keith A. Rodvoldb and Susan L. Pendlandb,*

,a Department of Veterans Affairs, Cooperative Studies Program, Clinical Research Pharmacy Coordinating Center, 2401 Center Avenue SE, Albuquerque, NM 87106-4180; ,bUniversity of Illinois at Chicago, Colleges of Pharmacy and Medicine, Department of Pharmacy Practice, 833 South Wood Street, Chicago, IL 60612, USA

This study used a modified time–kill assay to compare the in-vitro activity of chloramphenicol and quinopristin/dalfopristin combined with vancomycin, ampicillin or gentamicin against multidrug-resistant Enterococcus faecium. The assay uses standardized time–kill methods with the following modifications: centrifugation of the test tubes at 1–2 h intervals, removal of supernatant and resuspension of bacteria in media containing antibiotic concentrations corresponding to simulated steady-state serum concentrations. None of the agents, alone or in combination, produced bactericidal or synergic activity. The modified time–kill assay more closely simulates in-vivo conditions and may provide a better qualitative assay to determine the interaction between antimicrobial agents and bacteria.

* Corresponding author. Tel: +1-312-996-8639; Fax: +1-312-413-1797; E-mail: pendland{at}uic.edu


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 The Annals of PharmacotherapyHome page
J. Brown and B. B Freeman III
Combining Quinupristin/Dalfopristin with Other Agents for Resistant Infections
Ann. Pharmacother., April 1, 2004; 38(4): 677 - 685.
[Abstract] [Full Text] [PDF]


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