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Journal of Antimicrobial Chemotherapy (1999) 44, 377-380
© 1999 The British Society for Antimicrobial Chemotherapy

Brief report

Molecular characterization of a multiply resistant Klebsiella pneumoniae encoding ESBLs and a plasmid-mediated AmpC

Nancy D. Hanson*, Kenneth S. Thomson, Ellen Smith Moland, Christine C. Sanders, Gerald Berthold and Robert G. Penn

Center for Research in Anti-Infectives and Biotechnology,Departments of Pediatrics and Medical Microbiology and Immunology, Creighton University School of Medicine and Methodist Hospital, 2500 California Plaza, Omaha, NE 68178, USA

Organisms encoding multiple antibiotic resistance genes are becoming increasingly prevalent. In this report we describe a multiply resistant Klebsiella pneumoniae which possesses at least five different ß-lactamase genes. Isoelectric focusing, polymerase chain reaction and restriction fragment length polymorphism analysis identified TEM-1, multiple SHVs, OXA-9 and a plasmid-mediated ampC ß-lactamase. Furthermore, Southern analysis and conjugation experiments established that most of the resistance genes were encoded on one large transferable plasmid. This report demonstrates the complexity of multiply resistant organisms.

Corresponding author. Tel: +1-402-280-1881; Fax: +1-402-280-1225; E-mail: ndhanson{at}creighton.edu


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