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Journal of Antimicrobial Chemotherapy (1999) 43, 333-338
© 1999 The British Society for Antimicrobial Chemotherapy

For debate

Personalized antifungal susceptibility testing

Stefania Conti, Franco Fanti, Daniela Bertolotti, Elisabetta Dieci, Simona Arseni, Antonella Salati and Luciano Polonelli*

Istituto di Microbiologia, Facoltà di Medicina e Chirurgia, Università degli Studi di Parma, Via Gramsci 14, 43100 Parma, Italia

Introduction

In recent years, the remarkable increase in the number of infections caused by fungal pathogens, especially in immunocompromised people, has stimulated interest in medical mycology in general and in antifungal susceptibility testing in particular.1,2 In-vitro procedures for determining the activity of drugs against mould and yeast isolates involve clinicians in the choice and monitoring of antifungal chemotherapy. A number of factors, however, may influence the evaluation of the sensitivity of fungi to antifungal drugs, even in the most standardized procedures such as the determination of the MIC and minimal fungicidal concentration (MFC) in optically read microautomated systems. The nature and the form of the involved fungus, the preparation of the inoculum, the solubility and stability of the antifungal drugs tested, the pH and composition of the test medium, the duration and the temperature of incubation and the criteria for determining endpoints are variables that significantly affect the reproducibility of the . . . [Full Text of this Article]

Materials and methods

Yeast strains

Antifungal drugs

Synthetic medium and human plasma

Microtitre plate drug dilutions

Preparation of the inoculum

Interpretation of the results

Results

Discussion

Acknowledgments

Notes

References


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