Journal of Antimicrobial Chemotherapy, Vol 40, 511-516, Copyright © 1997 by The British Society for Antimicrobial Chemotherapy
JL May, A King and CA Warren
The increasing incidence of resistance to the antifungal agent,
fluconazole, prompted the need for a rapid, reliable and easy-to-use
susceptibility test. We have developed a disc diffusion test for
fluconazole against Candida spp. suitable for a clinical laboratory. Disc
diffusion tests on six different media were compared with MIC values. On
the basis of correlation coefficient with MICs (r = -0.95), quality of
growth and zone edge definition, Yeast Nitrogen Base agar with glucose
(YNBG) produced the best results. Further studies on YNBG showed that the
method is reliable for Candida albicans and for resistant isolates with no
zone of inhibition, but results for the slower growing and uncommon species
must be interpreted with some caution. Implementation of this test in the
clinical laboratory has provided a much needed therapeutic service for
clinicians within the hospital. It has also reduced the reliance on the
reference laboratory for susceptibility results and the consequent costs
involved.
ORIGINAL ARTICLES
Fluconazole disc diffusion testing for the routine laboratory
Department of Microbiology, UMDS, London, UK.
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