Journal of Antimicrobial Chemotherapy, Vol 40, 221-226, Copyright © 1997 by The British Society for Antimicrobial Chemotherapy
K Fuursted, A Hjort and L Knudsen
Lag of regrowth or postantibiotic effect (PAE) relates to suppression of
bacterial regrowth following short exposure to an antimicrobial agent. A
delay in regrowth has not yet been studied for antiseptics to any great
extent. We therefore examined and compared the lag of regrowth and the
bactericidal activity of five antiseptics (chloramine T, chlorhexidine,
povidone-iodine, phenoxyethanol and mandelic-lactic acid) against nine
bacterial pathogens. Delay in regrowth was determined by application of two
concentration-time schedules: a test concentration at the MBC with a
contact time of 1 h or using fixed suboptimum concentration of each
antiseptic for 2 min (optimum concentrations sterilized the culture,
impeding assessment of regrowth) followed by a neutralization-dilution step
and subsequent viable counting to follow bacterial regrowth. Each
antiseptic displayed a different spectrum of activity in terms of MIC or
MBC, bactericidal effect and lag of regrowth. The delay in regrowth varied
from 0 to 5.7 h with only a few discrepancies between the two treatment
schedules. Mandelic-lactic acid and chloramine T induced a significantly
longer lag as compared with the other agents, whereas phenoxyethanol
produced the shortest lag values. No significant correlation between the
killing rate and the lag of regrowth could be demonstrated. Information on
bactericidal activity, as well as lag of regrowth, could be a useful
screening method for the efficacy of antiseptics. Moreover, data on lag of
regrowth could contribute to the choice of antiseptic and guide in
determining the optimum interval between repeated applications of
antiseptics.
ORIGINAL ARTICLES
Evaluation of bactericidal activity and lag of regrowth (postantibiotic effect) of five antiseptics on nine bacterial pathogens
Department of Research and Development in Microbiology, Statens Serum Institut, Copenhagen S, Denmark.
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