Journal of Antimicrobial Chemotherapy, Vol 39, 737-745, Copyright © 1997 by The British Society for Antimicrobial Chemotherapy
M Leung, K Shannon and G French
We report a survey of beta-lactamases and their transferability in
Klebsiella spp. isolated from blood during 1992-95. beta-Lactamases were
characterized by determination of isoelectric point (pI), by hybridization
of plasmid DNA preparations with probes for SHV and TEM sequences and by
PCR with SHV- or TEM-specific primers. There were 80 isolates of Klebsiella
pneumoniae and 22 isolates of Klebsiella oxytoca. Most isolates of K.
pneumoniae had a chromosomally encoded SHV- 1 beta-lactamase (or a closely
related enzyme); K. oxytoca also produced chromosomal beta-lactamases, but
these were distinct from SHV- 1. Plasmid-encoded beta-lactamases were rare
in Klebsiella spp., being found in six (7.5%) isolates of K. pneumoniae and
in none of the K. oxytoca. beta-Lactamase activities were relatively low
(< 100 nmoles nitrocefin hydrolysed per minute per mg of protein) and
ampicillin MICs were < or = 128 mg/L for most isolates of both species.
However, all isolates of K. pneumoniae with plasmid-encoded
beta-lactamases, three other isolates of K. pneumoniae and three isolates
of K. oxytoca had high beta-lactamase activities (> 100 nmoles/mg/min)
and very high ampicillin MICs (> or = 1024 mg/L).
JOURNAL ARTICLE
Rarity of transferable beta-lactamase production by Klebsiella species
Department of Microbiology, UMDS, St Thomas's Campus, London, UK.
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