Journal of Antimicrobial Chemotherapy, Vol 39, 209-216, Copyright © 1997 by The British Society for Antimicrobial Chemotherapy
TQ Tan, CW Smith, EP Hawkins and SL Kaplan
Five-day-old infant rats were injected intraperitoneally (i.p.) with
anti-CD11b monoclonal antibody (1 B6) at a dose of 2 mg/kg or phosphate-
buffered saline (PBS) either 1 h before or 3 or 24 h after inoculation with
10(5) cfu Haemophilus influenzae type b (Hib). When administered 1 h before
infection, 23% of the 1B6- versus 17% of the PBS-treated rats and 87% of
the 1B6- versus 83% of the PBS-treated animals died at 24 and 48 h,
respectively. There was a similar mortality for 1B6 or PBS treatment at 3 h
after infection. Thirteen of 15 (87%) 1B6 animals versus 16/17 (94%) PBS
animals had positive CSF cultures at 48 h. No differences in mortality were
observed in separate experiments where animals received 1B6 or PBS 3 or 24
h after infection with Hib and were treated with a single ampicillin dose
(100 mg/kg) 24 h after infection. The median CSF white blood cell count/mm3
was 5627 and 4860 for the animals with meningitis receiving 1B6 and PBS,
respectively, although the 1B6-treated animals had a lower percentage of
polymorphonuclear cells in the CSF (P = 0.05). Histologic examination of
the meninges, choroid plexus and cochlea showed a slight decrease in the
numbers of inflammatory cells in animals treated with 1B6. 1B6 did not
change the incidence of meningitis and only slightly decreased the degree
of inflammation within the central nervous system, although animals treated
with 1B6 have an altered CSF leucocyte response with the presence of more
mononuclear cells as opposed to polymorphonuclear cells in their CSF. 1B6
may play a role in inhibiting neutrophil emigration to sites of
inflammation within the central nervous system but is not beneficial in
decreasing mortality in an infant rat model of H. influenzae type b sepsis
and meningitis.
JOURNAL ARTICLE
Anti-CD11b monoclonal antibody in an infant rat model of Haemophilus influenzae type b sepsis and meningitis
Department of Pediatrics, Baylor College of Medicine and Texas Children's Hospital, Houston 77030, USA.
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