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Journal of Antimicrobial Chemotherapy (1995) 35, 429-433
© 1995 The British Society for Antimicrobial Chemotherapy

brief-report

An enzymatic method for assaying sulbactam in human serum: comparison with high performance liquid chromatography

Albert Sottoa, Pascale Peraya, Florence Genyb, Caroline Brunschwiga, Christian Carrièrec, Marc Galtierb, Michel Ramuzc and Jacques Jourdana

aLaboratoire Universitaire de Thérapeutique, Service de Médecine B 30900 Nîmes, France bDépartement de Pharmacocinétique, Hôpital Caremeau, Rue du Professeur Debré 30900 Nîmes, France cLaboratoire de Microbiologie, Hôpital Gas ton Doumergue Rue Hoche, 30900 Nîmes, France

Received 28 February 1994; returned 5 August 1994; accepted 15 October 1994

An enzymatic method using nitrocefin as substrate was developed to assay sulbactam in human serum. Serum containing sulbactam was incubated with purified titrated TEM-1 ß-lactamase and nitrocefin was then added to the mixture to determine the remaining ß-lactamase activity and consequently the concentration of sulbactam. Assays were carried out on five patients with pulmonary infections receiving sulbactam plus amoxycillin iv. The values for serum sulbactam concentrations determined by the enzymatic method were compared with those determined by high performance liquid chromatography (HPLC). The correlation coefficient was 0·990 for serum sulbactam concentrations below 15 mg/L.


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A. Sotto, V. Foulongne, D. Sirot, and J. Jourdan
A method using TEM-5-{beta}-lactamase to assay imipenem
J. Antimicrob. Chemother., December 1, 1999; 44(6): 851 - 852.
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