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Journal of Antimicrobial Chemotherapy (1994) 34, 707-714
© 1994 The British Society for Antimicrobial Chemotherapy

research-article

Frequency of inhibitor-resistant TEM ß-lactamases in Escherichia coli isolates from urinary tract infections in Fran

C. Henquella, D. Sirota, C. Chanala, C. De Champsa, P. Chatronb, B. Lafeuillec, P. Texierd, J. Sirofa and R. Cluzela

aLaboratoire de Bacteriologie, Faculte de Medecine 28, place Henri Dunant, 63001 Clermont-Ferrand bLaboratoire d'Analyses de Biologie Medicale Monier-Chatron cLaboratoire d'Analyses de Biologie Médicale Chambon dLaboratoire d'Analyses de Biologie Médicale Texier, 63000 Clermont-Ferrand, France

Received 20 April 1994; accepted 6 August 1994

Over a six-month period in 1993, 2972 non-duplicated isolates of Escherichia coli causing urinary tract infections were collected in a French teaching hospital (n = 785) and in three private laboratories (n =2187). The resistance rate to amoxycillin-clavulanate combination (MIC > 16mg/l) was 25.0% in the hospital isolates and 100% in the community isolates. Respectively, 27.5% and 45.0% of hospital and community isolates resistant to amoxycillin-clavulanate exhibited an unusual ß-lactam resistance pattern, suggesting inhibitor-resistant TEM (IRT) >-lactamase production. These isolates were highly resistant to amoxycillin-clavulanate (MIC90 1024 mg/L), but were susceptible to cephalosporins (MIC < 32 mg/L). Enzyme extracts of these IRT-producing strains focused at pi 5.2 (n = 100) or 5.4 (n = 53). DNA-DNA hybridization confirmed that the ß-lactamases involved in this resistance mechanism were TEM-1 derived and contained variations in the altered positions described in IRT enzymes. This study shows a total frequency of 4.9% of IRT-producing isolates among E. coli isolated from urine specimens.


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